Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication

Kjersti M. Aagaard, Anismrita Lahon, Melissa A. Suter, Ravi P. Arya, Maxim D. Seferovic, Megan B. Vogt, Min Hu, Fabio Stossi, Michael A. Mancini, R. Alan Harris, Maike Kahr, Catherine Eppes, Martha Rac, Michael A. Belfort, Chun Shik Park, Daniel Lacorazza, Rebecca Rico-Hesse

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Zika virus (ZIKV) is an emerging mosquito-borne (Aedes genus) arbovirus of the Flaviviridae family. Although ZIKV has been predominately associated with a mild or asymptomatic dengue-like disease, its appearance in the Americas has been accompanied by a multi-fold increase in reported incidence of fetal microcephaly and brain malformations. The source and mode of vertical transmission from mother to fetus is presumptively transplacental, although a causal link explaining the interval delay between maternal symptoms and observed fetal malformations following infection has been missing. In this study, we show that primary human placental trophoblasts from non-exposed donors (n = 20) can be infected by primary passage ZIKV-FLR isolate, and uniquely allowed for ZIKV viral RNA replication when compared to dengue virus (DENV). Consistent with their being permissive for ZIKV infection, primary trophoblasts expressed multiple putative ZIKV cell entry receptors, and cellular function and differentiation were preserved. These findings suggest that ZIKV-FLR strain can replicate in human placental trophoblasts without host cell destruction, thereby serving as a likely permissive reservoir and portal of fetal transmission with risk of latent microcephaly and malformations.

Original languageEnglish (US)
Article number41389
JournalScientific Reports
Volume7
DOIs
StatePublished - Jan 27 2017
Externally publishedYes

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Trophoblasts
Virus Replication
Microcephaly
Flaviviridae
Arboviruses
Virus Internalization
Dengue Virus
Dengue
Aedes
Viral RNA
Culicidae
Zika Virus
Fetus
Mothers
Incidence
Brain
Infection

ASJC Scopus subject areas

  • General

Cite this

Aagaard, K. M., Lahon, A., Suter, M. A., Arya, R. P., Seferovic, M. D., Vogt, M. B., ... Rico-Hesse, R. (2017). Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Scientific Reports, 7, [41389]. https://doi.org/10.1038/srep41389

Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. / Aagaard, Kjersti M.; Lahon, Anismrita; Suter, Melissa A.; Arya, Ravi P.; Seferovic, Maxim D.; Vogt, Megan B.; Hu, Min; Stossi, Fabio; Mancini, Michael A.; Harris, R. Alan; Kahr, Maike; Eppes, Catherine; Rac, Martha; Belfort, Michael A.; Park, Chun Shik; Lacorazza, Daniel; Rico-Hesse, Rebecca.

In: Scientific Reports, Vol. 7, 41389, 27.01.2017.

Research output: Contribution to journalArticle

Aagaard, KM, Lahon, A, Suter, MA, Arya, RP, Seferovic, MD, Vogt, MB, Hu, M, Stossi, F, Mancini, MA, Harris, RA, Kahr, M, Eppes, C, Rac, M, Belfort, MA, Park, CS, Lacorazza, D & Rico-Hesse, R 2017, 'Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication', Scientific Reports, vol. 7, 41389. https://doi.org/10.1038/srep41389
Aagaard KM, Lahon A, Suter MA, Arya RP, Seferovic MD, Vogt MB et al. Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. Scientific Reports. 2017 Jan 27;7. 41389. https://doi.org/10.1038/srep41389
Aagaard, Kjersti M. ; Lahon, Anismrita ; Suter, Melissa A. ; Arya, Ravi P. ; Seferovic, Maxim D. ; Vogt, Megan B. ; Hu, Min ; Stossi, Fabio ; Mancini, Michael A. ; Harris, R. Alan ; Kahr, Maike ; Eppes, Catherine ; Rac, Martha ; Belfort, Michael A. ; Park, Chun Shik ; Lacorazza, Daniel ; Rico-Hesse, Rebecca. / Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication. In: Scientific Reports. 2017 ; Vol. 7.
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