Prevalence of the GABA(A) receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration

Ashok K. Mehta, Maharaj K. Ticku

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The anti-α1 antibody elicited higher immunoprecipitation (%) values of the [3H]flunitrazepam and [3H]muscimol binding activity in the rat cerebellum vs. cerebral cortex, whereas immunoprecipitation values for [3H]Ro 15-4513 and [3H]zolpidem were comparable in these brain regions. Chronic ethanol administration neither changed the radioligand binding to the immunoprecipitated pellet nor the percentage immunoprecipitation values, thereby indicating that chronic ethanol did not result in down-regulation of the GABA(A) receptor assemblies containing α1-subunit.

Original languageEnglish (US)
Pages (from-to)194-199
Number of pages6
JournalMolecular Brain Research
Volume67
Issue number1
DOIs
StatePublished - Apr 6 1999

Keywords

  • Anti-α antibody
  • Chronic ethanol
  • GABA(A) receptor
  • Immunoprecipitation
  • Radioligand binding

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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