Prevalence and clonal distribution of pcpA, psrP and pilus-1 among pediatric isolates of streptococcus pneumoniae

Laura Selva, Pilar Ciruela, Krystle Blanchette, Eva del Amo, Roman Pallares, Carlos J. Orihuela, Carmen Muñoz-Almagro

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19 Scopus citations

Abstract

Streptococcus pneumoniae is the leading cause of vaccine-preventable deaths globally. The objective of this study was to determine the distribution and clonal type variability of three potential vaccine antigens: Pneumococcal serine-rich repeat protein (PsrP), Pilus-1, and Pneumococcal choline binding protein A (PcpA) among pneumococcal isolates from children with invasive pneumococcal disease and healthy nasopharyngeal carriers. We studied by Real-Time PCR a total of 458 invasive pneumococcal isolates and 89 nasopharyngeal pneumococcal isolates among children (total = 547 strains) collected in Barcelona, Spain, from January 2004 to July 2010. pcpA, psrP and pilus-1 were detected in 92.8%, 51.7% and 14.4% of invasive isolates and in 92.1%, 48.3% and 18% of carrier isolates, respectively. Within individual serotypes the prevalence of psrP and pilus-1 was highly dependent on the clonal type. pcpA was highly prevalent in all strains with the exception of those belonging to serotype 3 (33.3% in serotype 3 isolates vs. 95.1% in other serotypes; P<.001). psrP was significantly more frequent in those serotypes that are less apt to be detected in carriage than in disease; 58.7% vs. 39.1% P<.001. Antibiotic resistance was associated with the presence of pilus-1 and showed a negative correlation with psrP. These results indicate that PcpA, and subsequently Psrp and Pilus-1 together might be good candidates to be used in a next-generation of multivalent pneumococcal protein vaccine.

Original languageEnglish (US)
Article numbere41587
JournalPloS one
Volume7
Issue number7
DOIs
StatePublished - Jul 25 2012

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Selva, L., Ciruela, P., Blanchette, K., del Amo, E., Pallares, R., Orihuela, C. J., & Muñoz-Almagro, C. (2012). Prevalence and clonal distribution of pcpA, psrP and pilus-1 among pediatric isolates of streptococcus pneumoniae. PloS one, 7(7), [e41587]. https://doi.org/10.1371/journal.pone.0041587