Presynaptically expressed long-term potentiation increases multivesicular release at parallel fiber synapses

Vanessa A. Bender, Jason R Pugh, Craig E. Jahr

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

At a number of synapses, long-term potentiation (LTP) can be expressed by an increase in presynaptic strength, but it is unknown whether presynaptic LTP is expressed solely through an increase in the probability that a single vesicle is released or whether it can increase multivesicular release (MVR). Here, we show that presynaptic LTP decreases inhibition of AMPA receptor EPSCs by a low-affinity antagonist at parallel fiber-molecular layer interneuron (PF-MLI) synapses. This indicates that LTP induction results in larger glutamate concentration transients in the synaptic cleft, a result indicative of MVR, and suggests that MVR can be modified by long-term plasticity. A similar decrease in inhibition was observed when release probability (PR) was increased by forskolin, elevated extracellular Ca2+, and paired-pulse facilitation. Furthermore, we show that MVR may occur under baseline physiological conditions, as inhibition increased when PR was lowered by reducing extracellular Ca2+ or by activating presynaptic adenosine receptors. These results suggest that at PF-MLI synapses, MVR occurs under control conditions and is increased when PR is elevated by both short- and long-term plasticity mechanisms.

Original languageEnglish (US)
Pages (from-to)10974-10978
Number of pages5
JournalJournal of Neuroscience
Volume29
Issue number35
DOIs
StatePublished - Sep 2 2009
Externally publishedYes

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Long-Term Potentiation
Synapses
Interneurons
Presynaptic Receptors
Purinergic P1 Receptors
AMPA Receptors
Colforsin
Glutamic Acid

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Presynaptically expressed long-term potentiation increases multivesicular release at parallel fiber synapses. / Bender, Vanessa A.; Pugh, Jason R; Jahr, Craig E.

In: Journal of Neuroscience, Vol. 29, No. 35, 02.09.2009, p. 10974-10978.

Research output: Contribution to journalArticle

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