Presynaptic serotonin receptors regulate [3H]serotonin release from rat spinal cord synaptosomes

Kenneth A. Stauderman; David J. Jones

doi:10.1016/0014-2999(86)90647-3

Presynaptic serotonin receptors regulate [³H]serotonin release from rat spinal cord synaptosomes

Kenneth A. Stauderman, David J. Jones

Anesthesiology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Superfused rat spinal cord synaptosomes were studied to determine if inhibitory serotonin (5-HT) receptors (autoreceptors) exist on spinal serotonergic nerve terminals. Exogenous 5-HT (1-50 nM) produced a concentration-dependent inhibition of K⁺-induced [³H]5-HT release but did not affect basal [³H]5-HT release. A 32-44% inhibition was produced,by 30 nM 5-HT. The inhibitory effect of 30 nM 5-HT was effectively antagonized by 100 nM metitepine, a 5-HT autoreceptor antagonist. The results provide evidence for the existence of 5-HT autoreceptors in rat spinal cord tissue.

Original language	English (US)
Pages (from-to)	107-109
Number of pages	3
Journal	European Journal of Pharmacology
Volume	120
Issue number	1
DOIs	https://doi.org/10.1016/0014-2999(86)90647-3
State	Published - Jan 14 1986

Keywords

Autoreceptors
Metitepine
Serotonin
Spinal cord

ASJC Scopus subject areas

Pharmacology

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10.1016/0014-2999(86)90647-3

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title = "Presynaptic serotonin receptors regulate [3H]serotonin release from rat spinal cord synaptosomes",

abstract = "Superfused rat spinal cord synaptosomes were studied to determine if inhibitory serotonin (5-HT) receptors (autoreceptors) exist on spinal serotonergic nerve terminals. Exogenous 5-HT (1-50 nM) produced a concentration-dependent inhibition of K+-induced [3H]5-HT release but did not affect basal [3H]5-HT release. A 32-44% inhibition was produced,by 30 nM 5-HT. The inhibitory effect of 30 nM 5-HT was effectively antagonized by 100 nM metitepine, a 5-HT autoreceptor antagonist. The results provide evidence for the existence of 5-HT autoreceptors in rat spinal cord tissue.",

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AB - Superfused rat spinal cord synaptosomes were studied to determine if inhibitory serotonin (5-HT) receptors (autoreceptors) exist on spinal serotonergic nerve terminals. Exogenous 5-HT (1-50 nM) produced a concentration-dependent inhibition of K+-induced [3H]5-HT release but did not affect basal [3H]5-HT release. A 32-44% inhibition was produced,by 30 nM 5-HT. The inhibitory effect of 30 nM 5-HT was effectively antagonized by 100 nM metitepine, a 5-HT autoreceptor antagonist. The results provide evidence for the existence of 5-HT autoreceptors in rat spinal cord tissue.

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