Preparative fractionation of DNA restriction fragments by reversed phase column chromatography

S. C. Hardies, R. D. Wells

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Reversed phase column chromatography on RPC 5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undetermined property.

Original languageEnglish (US)
Pages (from-to)3117-3121
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume73
Issue number9
DOIs
StatePublished - Jan 1 1976

ASJC Scopus subject areas

  • General

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