Preparative fractionation of DNA restriction fragments by reversed phase column chromatography

S. C. Hardies; R. D. Wells

doi:10.1073/pnas.73.9.3117

Preparative fractionation of DNA restriction fragments by reversed phase column chromatography

S. C. Hardies, R. D. Wells

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Reversed phase column chromatography on RPC 5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undetermined property.

Original language	English (US)
Pages (from-to)	3117-3121
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	73
Issue number	9
DOIs	https://doi.org/10.1073/pnas.73.9.3117
State	Published - 1976

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title = "Preparative fractionation of DNA restriction fragments by reversed phase column chromatography",

abstract = "Reversed phase column chromatography on RPC 5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undetermined property.",

author = "Hardies, {S. C.} and Wells, {R. D.}",

year = "1976",

doi = "10.1073/pnas.73.9.3117",

language = "English (US)",

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AU - Wells, R. D.

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AB - Reversed phase column chromatography on RPC 5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undetermined property.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

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Preparative fractionation of DNA restriction fragments by reversed phase column chromatography

Abstract

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