Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay

J. H. Jorgensen; R. F. Smith

doi:10.1128/aem.26.1.43-48.1973

Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay

J. H. Jorgensen, R. F. Smith

Department of Pathology

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxin like activity was observed from 11 different strains of yeast by use of the Limulus assay.

Original language	English (US)
Pages (from-to)	43-48
Number of pages	6
Journal	Journal of Applied Microbiology
Volume	26
Issue number	1
DOIs	https://doi.org/10.1128/aem.26.1.43-48.1973
State	Published - 1973

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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10.1128/aem.26.1.43-48.1973

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AB - Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxin like activity was observed from 11 different strains of yeast by use of the Limulus assay.

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Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay

Abstract

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