Preparation, sensitivity, and specificity of Limulus lysate for endotoxin assay

J. H. Jorgensen, R. F. Smith

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Limulus amoebocyte lysate was prepared from a total of 180 crabs during 1971 and 1972 by using a slightly modified lysate preparation procedure. Marked variability of lysate potency was noted both years. In addition, lysate quality appeared diminished in 1972 as compared with 1971. Different lysate batches were evaluated for potency by using a variety of endotoxin preparations. Variations in batch potencies were observed, but little variation in reactivity among different endotoxin preparations was noted. Use of potent lysate batches allowed detection of endotoxin concentrations as low as 100 pg/ml. No endotoxin like activity was observed from 11 different strains of yeast by use of the Limulus assay.

Original languageEnglish (US)
Pages (from-to)43-48
Number of pages6
JournalJournal of Applied Microbiology
Issue number1
StatePublished - 1973

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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