Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues

Kang Ning, Hao Zhang, Ju Qiang Han, Hong Xue Fan, Jie Zhi Li, Rong Li, Qi Nong Ye

Research output: Contribution to journalArticlepeer-review

Abstract

AIM: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice. METHODS: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot. RESULTS: An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot. CONCLUSION: Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.

Original languageEnglish (US)
Pages (from-to)794-796
Number of pages3
JournalXi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Volume22
Issue number6
StatePublished - Nov 2006

ASJC Scopus subject areas

  • Medicine(all)

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