Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues

Kang Ning; Hao Zhang; Ju Qiang Han; Hong Xue Fan; Jie Zhi Li; Rong Li; Qi Nong Ye

Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues

Kang Ning, Hao Zhang, Ju Qiang Han, Hong Xue Fan, Jie Zhi Li, Rong Li, Qi Nong Ye

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

AIM: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice. METHODS: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot. RESULTS: An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot. CONCLUSION: Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.

Original language	English (US)
Pages (from-to)	794-796
Number of pages	3
Journal	Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Volume	22
Issue number	6
State	Published - Nov 2006

ASJC Scopus subject areas

Medicine(all)

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Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues. / Ning, Kang; Zhang, Hao; Han, Ju Qiang; Fan, Hong Xue; Li, Jie Zhi; Li, Rong; Ye, Qi Nong.

In: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, Vol. 22, No. 6, 11.2006, p. 794-796.

Research output: Contribution to journal › Article › peer-review

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title = "Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues",

abstract = "AIM: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice. METHODS: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot. RESULTS: An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot. CONCLUSION: Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.",

author = "Kang Ning and Hao Zhang and Han, {Ju Qiang} and Fan, {Hong Xue} and Li, {Jie Zhi} and Rong Li and Ye, {Qi Nong}",

year = "2006",

month = nov,

language = "English (US)",

volume = "22",

pages = "794--796",

journal = "Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology",

issn = "1007-8738",

publisher = "Fourth Military Medical University",

number = "6",

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TY - JOUR

T1 - Preparation of antibody against Memo protein and analysis of expression profile of Memo in mouse tissues

AU - Ning, Kang

AU - Zhang, Hao

AU - Han, Ju Qiang

AU - Fan, Hong Xue

AU - Li, Jie Zhi

AU - Li, Rong

AU - Ye, Qi Nong

PY - 2006/11

Y1 - 2006/11

N2 - AIM: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice. METHODS: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot. RESULTS: An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot. CONCLUSION: Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.

AB - AIM: To prepare and characterize antibody against Memo protein and to detect the tissue distribution of Memo in mice. METHODS: Fusion protein GST-Memo was expressed and purified, and polyclonal antibody against Memo was prepared by immunizing mice. A FLAG-tagged eukaryotic expression vector pcDNA3-FLAG-Memo was constructed. The specificity of the antibody was detected by Western blot. RESULTS: An eukaryotic expression vector pcDNA3-FLAG-Memo was obtained. The polyclonal antibody was found to be specific to Memo. Memo protein was widely expressed in mouse tissues using the obtained antibody in Western blot. CONCLUSION: Antibody specific to Memo has been successfully obtained, which provides useful tool for investigation into Memo-associated mechanisms of tumor metastasis and invasiveness.

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