Preparation and application of monoclonal antibody against hNDRG2

Xinping Liu; Xiaolan Hu; Jian Zhang; Lifeng Wang; Wenghong Zhang; Xuesong Liu; Fuyang Li; Yuanqiang Zhang; Libo Yao

doi:10.1007/s12010-008-8267-3

Preparation and application of monoclonal antibody against hNDRG2

Xinping Liu
, Xiaolan Hu
, Jian Zhang
, Lifeng Wang
, Wenghong Zhang
, Xuesong Liu
, Fuyang Li
, Yuanqiang Zhang
, Libo Yao

Greehey Children's Cancer Research Institute

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

Original language	English (US)
Pages (from-to)	306-315
Number of pages	10
Journal	Applied Biochemistry and Biotechnology
Volume	152
Issue number	2
DOIs	https://doi.org/10.1007/s12010-008-8267-3
State	Published - Feb 2009

Keywords

Fusion protein
Immunohistochemistry
Monoclonal antibody against hNdrg2
N-myc downstream-regulated gene

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Bioengineering
Molecular Biology
Biochemistry
Biotechnology

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10.1007/s12010-008-8267-3

Cite this

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title = "Preparation and application of monoclonal antibody against hNDRG2",

abstract = "The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.",

keywords = "Fusion protein, Immunohistochemistry, Monoclonal antibody against hNdrg2, N-myc downstream-regulated gene",

author = "Xinping Liu and Xiaolan Hu and Jian Zhang and Lifeng Wang and Wenghong Zhang and Xuesong Liu and Fuyang Li and Yuanqiang Zhang and Libo Yao",

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TY - JOUR

T1 - Preparation and application of monoclonal antibody against hNDRG2

AU - Liu, Xinping

AU - Hu, Xiaolan

AU - Zhang, Jian

AU - Wang, Lifeng

AU - Zhang, Wenghong

AU - Liu, Xuesong

AU - Li, Fuyang

AU - Zhang, Yuanqiang

AU - Yao, Libo

PY - 2009/2

Y1 - 2009/2

N2 - The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

AB - The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

KW - Fusion protein

KW - Immunohistochemistry

KW - Monoclonal antibody against hNdrg2

KW - N-myc downstream-regulated gene

UR - https://www.scopus.com/pages/publications/58849098737

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DO - 10.1007/s12010-008-8267-3

M3 - Article

C2 - 18758694

AN - SCOPUS:58849098737

SN - 0273-2289

VL - 152

SP - 306

EP - 315

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 2

ER -

Preparation and application of monoclonal antibody against hNDRG2

Abstract

Keywords

ASJC Scopus subject areas

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