Preparation and application of monoclonal antibody against hNDRG2

Xinping Liu; Xiaolan Hu; Jian Zhang; Lifeng Wang; Wenghong Zhang; Xuesong Liu; Fuyang Li; Yuanqiang Zhang; Libo Yao

doi:10.1007/s12010-008-8267-3

Preparation and application of monoclonal antibody against hNDRG2

Xinping Liu, Xiaolan Hu, Jian Zhang, Lifeng Wang, Wenghong Zhang, Xuesong Liu, Fuyang Li, Yuanqiang Zhang, Libo Yao

Greehey Children's Cancer Research Institute

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

Original language	English (US)
Pages (from-to)	306-315
Number of pages	10
Journal	Applied Biochemistry and Biotechnology
Volume	152
Issue number	2
DOIs	https://doi.org/10.1007/s12010-008-8267-3
State	Published - Feb 2009

Keywords

Fusion protein
Immunohistochemistry
Monoclonal antibody against hNdrg2
N-myc downstream-regulated gene

ASJC Scopus subject areas

Applied Microbiology and Biotechnology
Bioengineering
Molecular Biology
Biochemistry
Biotechnology

Access to Document

10.1007/s12010-008-8267-3

Cite this

@article{59b7fdc18e6a489d9250c0b94948dba3,

title = "Preparation and application of monoclonal antibody against hNDRG2",

abstract = "The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.",

keywords = "Fusion protein, Immunohistochemistry, Monoclonal antibody against hNdrg2, N-myc downstream-regulated gene",

author = "Xinping Liu and Xiaolan Hu and Jian Zhang and Lifeng Wang and Wenghong Zhang and Xuesong Liu and Fuyang Li and Yuanqiang Zhang and Libo Yao",

year = "2009",

month = feb,

doi = "10.1007/s12010-008-8267-3",

language = "English (US)",

volume = "152",

pages = "306--315",

journal = "Applied Biochemistry and Biotechnology",

issn = "0273-2289",

publisher = "Humana Press",

number = "2",

}

TY - JOUR

T1 - Preparation and application of monoclonal antibody against hNDRG2

AU - Liu, Xinping

AU - Hu, Xiaolan

AU - Zhang, Jian

AU - Wang, Lifeng

AU - Zhang, Wenghong

AU - Liu, Xuesong

AU - Li, Fuyang

AU - Zhang, Yuanqiang

AU - Yao, Libo

PY - 2009/2

Y1 - 2009/2

N2 - The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

AB - The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

KW - Fusion protein

KW - Immunohistochemistry

KW - Monoclonal antibody against hNdrg2

KW - N-myc downstream-regulated gene

UR - http://www.scopus.com/inward/record.url?scp=58849098737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58849098737&partnerID=8YFLogxK

U2 - 10.1007/s12010-008-8267-3

DO - 10.1007/s12010-008-8267-3

M3 - Article

C2 - 18758694

AN - SCOPUS:58849098737

SN - 0273-2289

VL - 152

SP - 306

EP - 315

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 2

ER -

Preparation and application of monoclonal antibody against hNDRG2

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this