Preparation and application of monoclonal antibody against hNDRG2

Xinping Liu, Xiaolan Hu, Jian Zhang, Lifeng Wang, Wenghong Zhang, Xuesong Liu, Fuyang Li, Yuanqiang Zhang, Libo Yao

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The full-length hNdrg2 cDNA-coded 357 amino acids was cloned and expressed in Escherichia coli strain DH5α as a 6× His-tagged protein. The purified 6× His-fusion protein was used to immunize mice for preparing monoclonal antibodies (mAb) against N-myc downstream-regulated gene 2 (Ndrg2). A hybridoma secreting a monoclonal antibody against Ndrg2 was obtained and named FMU-Ndrg2.3. Western blot analysis confirmed that this mAb is specific only to Ndrg2 but not to Ndrg1, Ndrg3, and Ndrg4-B. Some tissue distribution features of Ndrg2 proteins, such as thyroid, kidney, testis, prostate, and pancreas islets, were present by immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)306-315
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume152
Issue number2
DOIs
StatePublished - Feb 2009

Keywords

  • Fusion protein
  • Immunohistochemistry
  • Monoclonal antibody against hNdrg2
  • N-myc downstream-regulated gene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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