Abstract
The five predominant types of rDNA repeats in D. melanogaster were analyzed with respect to their DNase I sensitivity. Only the insert-free repeats showed a generalized DNase I sensitivity pattern whereas the major type I, both minor type I and type II repeats were not as extensively degraded by the nuclease. For XX and XY embryonic nuclei, where there is rapid cell division, the majority of the In- repeats were DNase I sensitive. This indicated that these In- repeats have the potential to be transcribed during this developmental stage. When compared to the In- repeats, the chromatin configuration of the In+ repeats is indicative of a higher order of chromatin folding. The paucity of In+ primary gene transcripts observed in vivo could result from In+ repeats being packaged into a more condensed form of chromatin.
Original language | English (US) |
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Pages (from-to) | 2869-2879 |
Number of pages | 11 |
Journal | Nucleic acids research |
Volume | 13 |
Issue number | 8 |
DOIs | |
State | Published - Apr 25 1985 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics