TY - JOUR
T1 - Predictive models of insulin resistance derived from simple morphometric and biochemical indices related to obesity and the metabolic syndrome in baboons
AU - Chavez, Alberto O.
AU - Gastaldelli, Amalia
AU - Guardado-Mendoza, Rodolfo
AU - Lopez-Alvarenga, Juan C.
AU - Michelle, M. Michelle
AU - Elizabeth, M. Elizabeth
AU - Sorice, Gian Pio
AU - Casiraghi, Francesca
AU - Davalli, Alberto
AU - Bastarrachea, Raúl A.
AU - Comuzzie, Anthony G.
AU - DeFronzo, Ralph A.
AU - Folli, Franco
N1 - Funding Information:
This work was supported in part by UTHSCSA startup funding (FF), the Kronkosky Charitable Foundation (RAB, AGC) and conducted in facilities constructed with support from the Research Facilities Improvement Program under grant numbers C06 RR014578, C06 RR013556, C06 RR015456, and C06 RR017515 from the National Center for Research Resources of the National Institutes of Health, and the Primate Center grant P51RR013986. AOC is supported by a Mentor Based Postdoctoral Fellowship awarded to RAD by the American Diabetes Association. FC is partially supported by a fellowship "Borsa di Studio di Perfezionamento all'Estero", Faculty of Exercise Science, University of Milan, Italy. GPS was partially supported by an Endocrinology Fellowship from Universita' Cattol-ica del Sacro Cuore Roma Italy. We thank Monica Palomo for her excellent assistance throughout the course of these studies.
PY - 2009/4/23
Y1 - 2009/4/23
N2 - Background: Non-human primates are valuable models for the study of insulin resistance and human obesity. In baboons, insulin sensitivity levels can be evaluated directly with the euglycemic clamp and is highly predicted by adiposity, metabolic markers of obesity and impaired glucose metabolism (i.e. percent body fat by DXA and HbA1c). However, a simple method to screen and identify obese insulin resistant baboons for inclusion in interventional studies is not available. Methods: We studied a population of twenty baboons with the euglycemic clamp technique to characterize a population of obese nondiabetic, insulin resistant baboons, and used a multivariate linear regression analysis (adjusted for gender) to test different predictive models of insulin sensitivity (insulin-stimulated glucose uptake = Rd) using abdominal circumference and fasting plasma insulin. Alternatively, we tested in a separate baboon population (n = 159), a simpler model based on body weight and fasting plasma glucose to predict the whole-body insulin sensitivity (Rd/SSPI) derived from the clamp. Results: In the first model, abdominal circumference explained 59% of total insulin mediated glucose uptake (Rd). A second model, which included fasting plasma insulin (log transformed) and abdominal circumference, explained 64% of Rd. Finally, the model using body weight and fasting plasma glucose explained 51% of Rd/SSPI. Interestingly, we found that percent body fat was directly correlated with the adipocyte insulin resistance index (r = 0.755, p < 0.0001). Conclusion: In baboons, simple morphometric measurements of adiposity/ obesity, (i.e. abdominal circumference), plus baseline markers of glucose/lipid metabolism, (i.e. fasting plasma glucose and insulin) provide a feasible method to screen and identify overweight/obese insulin resistant baboons for inclusion in interventional studies aimed to study human obesity, insulin resistance and type 2 diabetes mellitus.
AB - Background: Non-human primates are valuable models for the study of insulin resistance and human obesity. In baboons, insulin sensitivity levels can be evaluated directly with the euglycemic clamp and is highly predicted by adiposity, metabolic markers of obesity and impaired glucose metabolism (i.e. percent body fat by DXA and HbA1c). However, a simple method to screen and identify obese insulin resistant baboons for inclusion in interventional studies is not available. Methods: We studied a population of twenty baboons with the euglycemic clamp technique to characterize a population of obese nondiabetic, insulin resistant baboons, and used a multivariate linear regression analysis (adjusted for gender) to test different predictive models of insulin sensitivity (insulin-stimulated glucose uptake = Rd) using abdominal circumference and fasting plasma insulin. Alternatively, we tested in a separate baboon population (n = 159), a simpler model based on body weight and fasting plasma glucose to predict the whole-body insulin sensitivity (Rd/SSPI) derived from the clamp. Results: In the first model, abdominal circumference explained 59% of total insulin mediated glucose uptake (Rd). A second model, which included fasting plasma insulin (log transformed) and abdominal circumference, explained 64% of Rd. Finally, the model using body weight and fasting plasma glucose explained 51% of Rd/SSPI. Interestingly, we found that percent body fat was directly correlated with the adipocyte insulin resistance index (r = 0.755, p < 0.0001). Conclusion: In baboons, simple morphometric measurements of adiposity/ obesity, (i.e. abdominal circumference), plus baseline markers of glucose/lipid metabolism, (i.e. fasting plasma glucose and insulin) provide a feasible method to screen and identify overweight/obese insulin resistant baboons for inclusion in interventional studies aimed to study human obesity, insulin resistance and type 2 diabetes mellitus.
UR - http://www.scopus.com/inward/record.url?scp=65149085747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65149085747&partnerID=8YFLogxK
U2 - 10.1186/1475-2840-8-22
DO - 10.1186/1475-2840-8-22
M3 - Article
C2 - 19389241
AN - SCOPUS:65149085747
SN - 1475-2840
VL - 8
JO - Cardiovascular Diabetology
JF - Cardiovascular Diabetology
M1 - 22
ER -