Predicted fold for the ABri amyloid subunit: A model for amyloidogenesis in Familial British Dementia

D. Mahadevan; T. Chattopadhyay; R. A. Palmer; R. O'Brien; J. W. Saldanha

doi:10.2174/0929866013409634

Predicted fold for the ABri amyloid subunit: A model for amyloidogenesis in Familial British Dementia

D. Mahadevan, T. Chattopadhyay, R. A. Palmer, R. O'Brien, J. W. Saldanha

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

ABri is a 34 residue polypeptide that forms amyloid fibrils in Familial British Dementia (FBD). A PSI-Blast search, 3 different fold recognition programs and 3D model-building indicated that it has 3 β-strands forming an antiparallel β-sheet and a small C-terminal α-helix. In order to validate the prediction, the region coding the ABri polypeptide was cloned, expressed and purified from E. Coli. Circular Dichroism (CD) spectroscopy of ABri in aqueous solution shows it to have a predominantly β-sheet structure with a small tx-helical component.

Original language	English (US)
Pages (from-to)	139-146
Number of pages	8
Journal	Protein and Peptide Letters
Volume	8
Issue number	2
DOIs	https://doi.org/10.2174/0929866013409634
State	Published - May 22 2001
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Biochemistry

Access to Document

10.2174/0929866013409634

Cite this

@article{321cbe5adc11436d9f52e862b9796848,

title = "Predicted fold for the ABri amyloid subunit: A model for amyloidogenesis in Familial British Dementia",

abstract = "ABri is a 34 residue polypeptide that forms amyloid fibrils in Familial British Dementia (FBD). A PSI-Blast search, 3 different fold recognition programs and 3D model-building indicated that it has 3 β-strands forming an antiparallel β-sheet and a small C-terminal α-helix. In order to validate the prediction, the region coding the ABri polypeptide was cloned, expressed and purified from E. Coli. Circular Dichroism (CD) spectroscopy of ABri in aqueous solution shows it to have a predominantly β-sheet structure with a small tx-helical component.",

author = "D. Mahadevan and T. Chattopadhyay and Palmer, {R. A.} and R. O'Brien and Saldanha, {J. W.}",

year = "2001",

month = may,

day = "22",

doi = "10.2174/0929866013409634",

language = "English (US)",

volume = "8",

pages = "139--146",

journal = "Protein and Peptide Letters",

issn = "0929-8665",

publisher = "Bentham Science Publishers B.V.",

number = "2",

}

TY - JOUR

T1 - Predicted fold for the ABri amyloid subunit

T2 - A model for amyloidogenesis in Familial British Dementia

AU - Mahadevan, D.

AU - Chattopadhyay, T.

AU - Palmer, R. A.

AU - O'Brien, R.

AU - Saldanha, J. W.

PY - 2001/5/22

Y1 - 2001/5/22

N2 - ABri is a 34 residue polypeptide that forms amyloid fibrils in Familial British Dementia (FBD). A PSI-Blast search, 3 different fold recognition programs and 3D model-building indicated that it has 3 β-strands forming an antiparallel β-sheet and a small C-terminal α-helix. In order to validate the prediction, the region coding the ABri polypeptide was cloned, expressed and purified from E. Coli. Circular Dichroism (CD) spectroscopy of ABri in aqueous solution shows it to have a predominantly β-sheet structure with a small tx-helical component.

AB - ABri is a 34 residue polypeptide that forms amyloid fibrils in Familial British Dementia (FBD). A PSI-Blast search, 3 different fold recognition programs and 3D model-building indicated that it has 3 β-strands forming an antiparallel β-sheet and a small C-terminal α-helix. In order to validate the prediction, the region coding the ABri polypeptide was cloned, expressed and purified from E. Coli. Circular Dichroism (CD) spectroscopy of ABri in aqueous solution shows it to have a predominantly β-sheet structure with a small tx-helical component.

UR - http://www.scopus.com/inward/record.url?scp=0035014783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035014783&partnerID=8YFLogxK

U2 - 10.2174/0929866013409634

DO - 10.2174/0929866013409634

M3 - Article

AN - SCOPUS:0035014783

SN - 0929-8665

VL - 8

SP - 139

EP - 146

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 2

ER -

Predicted fold for the ABri amyloid subunit: A model for amyloidogenesis in Familial British Dementia

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this