TY - JOUR
T1 - Pre- and/or postnatal protein restriction in rats impairs learning and motivation in male offspring
AU - Reyes-Castro, L. A.
AU - Rodriguez, J. S.
AU - Rodríguez-González, G. L.
AU - Wimmer, R. D.
AU - McDonald, T. J.
AU - Larrea, F.
AU - Nathanielsz, P. W.
AU - Zambrano, E.
N1 - Funding Information:
L Reyes-Castro and GL Rodriguez-Gonzalez are graduate students from Doctorado en Ciencias Biomédicas, Facultad de Medicina, Universidad Nacional Autónoma de México and are recipients of CONACyT fellowship. This work was partially supported by Consejo Nacional de Ciencia y Tecnología (CONACyT - 48839) México and the NIH HD21350.
PY - 2011/4
Y1 - 2011/4
N2 - Suboptimal developmental environments program offspring to lifelong health complications including affective and cognitive disorders. Little is known about the effects of suboptimal intra-uterine environments on associative learning and motivational behavior. We hypothesized that maternal isocaloric low protein diet during pregnancy and lactation would impair offspring associative learning and motivation as measured by operant conditioning and the progressive ratio task, respectively. Control mothers were fed 20% casein (C) and restricted mothers (R) 10% casein to provide four groups: CC, RR, CR, and RC (first letter pregnancy diet and second letter lactation diet), to evaluate effects of maternal diet on male offspring behavior. Impaired learning was observed during fixed ratio - 1 operant conditioning in RC offspring that required more sessions to learn vs. the CC offspring (9.4 ± 0.8 and 3.8 ± 0.3 sessions, respectively, p< 0.05). Performance in fixed ratio - 5 conditioning showed the RR (5.4 ± 1.1), CR (4.0 ± 0.8), and RC (5.0 ± 0.8) offspring required more sessions to reach performance criterion than CC offspring (2.5 ± 0.5, p< 0.05). Furthermore, motivational effects during the progressive ratio test revealed less responding in the RR (48.1 ± 17), CR (74.7 ± 8.4), and RC (65.9 ± 11.2) for positive reinforcement vs. the CC offspring (131.5 ± 7.5, p< 0.05). These findings demonstrate negative developmental programming effects due to perinatal isocaloric low protein diet on learning and motivation behavior with the nutritional challenge in the prenatal period showing more vulnerability in offspring behavior.
AB - Suboptimal developmental environments program offspring to lifelong health complications including affective and cognitive disorders. Little is known about the effects of suboptimal intra-uterine environments on associative learning and motivational behavior. We hypothesized that maternal isocaloric low protein diet during pregnancy and lactation would impair offspring associative learning and motivation as measured by operant conditioning and the progressive ratio task, respectively. Control mothers were fed 20% casein (C) and restricted mothers (R) 10% casein to provide four groups: CC, RR, CR, and RC (first letter pregnancy diet and second letter lactation diet), to evaluate effects of maternal diet on male offspring behavior. Impaired learning was observed during fixed ratio - 1 operant conditioning in RC offspring that required more sessions to learn vs. the CC offspring (9.4 ± 0.8 and 3.8 ± 0.3 sessions, respectively, p< 0.05). Performance in fixed ratio - 5 conditioning showed the RR (5.4 ± 1.1), CR (4.0 ± 0.8), and RC (5.0 ± 0.8) offspring required more sessions to reach performance criterion than CC offspring (2.5 ± 0.5, p< 0.05). Furthermore, motivational effects during the progressive ratio test revealed less responding in the RR (48.1 ± 17), CR (74.7 ± 8.4), and RC (65.9 ± 11.2) for positive reinforcement vs. the CC offspring (131.5 ± 7.5, p< 0.05). These findings demonstrate negative developmental programming effects due to perinatal isocaloric low protein diet on learning and motivation behavior with the nutritional challenge in the prenatal period showing more vulnerability in offspring behavior.
KW - Developmental programming
KW - Learning
KW - Motivation
KW - Operant conditioning
KW - Protein restriction
UR - http://www.scopus.com/inward/record.url?scp=79951579338&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951579338&partnerID=8YFLogxK
U2 - 10.1016/j.ijdevneu.2010.11.002
DO - 10.1016/j.ijdevneu.2010.11.002
M3 - Article
C2 - 21078378
AN - SCOPUS:79951579338
VL - 29
SP - 177
EP - 182
JO - International Journal of Developmental Neuroscience
JF - International Journal of Developmental Neuroscience
SN - 0736-5748
IS - 2
ER -