Practical guide for constructing a pulse compressor used in multi-photon microscopy

J. D. Lechleiter, D. T. Lin, I. Sieneart

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


Multi-photon imaging has generated intense interest from investigators using fluorescent imaging assays in live cells. It can be argued that this technique is rapidly overtaking confocal microscopy as the method of choice for three-dimensional fluorescent imaging of in vivo preparations. Because of the cost involved in purchasing a commercial multi-photon imaging microscope, many investigators have elected to build their own system by adapting in-house confocal laser scanning microscopes. One of the components used for this adaptation involves the construction of an external pulse compressor. Pulse compressors are used to add negative dispersion to pulsed radiation, which undergoes group velocity dispersion when traversing optical elements. In this chapter, we describe a practical guide to building an external pulse compressor.

Original languageEnglish (US)
Pages (from-to)111-117
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - 2001
EventMultiphoton Microscopy in the Biomedical Sciences - San Jose, CA, United States
Duration: Jan 21 2001Jan 23 2001


  • Group velocity dispersion
  • Multi-photon imaging
  • Pulse compressor
  • Pulsed radiation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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