@article{2aa0710d3d8d4c34a2317e5a35643f5c,
title = "PP2C phosphatases Ptc2 and Ptc3 are required for DNA checkpoint inactivation after a double-strand break",
abstract = "Saccharomyces cells suffering a DNA double-strand break (DSB) ultimately escape checkpoint-mediated G2/M arrest either by recovery once the lesion is repaired or by adaptation if the lesion proves irreparable. Cells lacking the PP2C-like phosphatases Ptc2 and Ptc3 are unable to adapt to a HO-induced DSB and are also defective in recovering from a repairable DSB. In contrast, overexpression of PTC2 rescues adaptation-defective yku80Δ and cdc5-ad mutants. These effects are not explained by alterations either in the processing of DSB ends or in DSB repair. In vivo and in vitro evidence suggests that phosphorylated forms of Ptc2 and Ptc3 specifically bind to the Rad53 FHA1 domain and inactivate Rad53-dependent pathways during adaptation and recovery by dephosphorylating Rad53.",
author = "Christophe Leroy and Lee, {Sang Eun} and Vaze, {Moreshwar B.} and Fran{\c c}oise Ochsenbien and Rapha{\"e}l Guerois and Haber, {James E.} and Marsolier-Kergoat, {Marie Claude}",
note = "Funding Information: We wish to thank S. Marcand for the gift of strains and for discussions, members of the Haber lab for discussions, S. Mauger for technical assistance, and A.A. Welihinda, R.J. Kaufman, J.F.X. Diffley, and D. Stern for providing reagents. This work was financed in part by a specific radiobiology action grant from the Minist{\`e}re de l'Education Nationale, de la Recherche et de la Technologie, and by the Association pour la Recherche sur le Cancer. C.L. is supported by an EDF/CEA fellowship. Research was also supported by NIH grants GM20056 and GM61766, and DOE grant 99ER62729 to J.E.H. S.E.L. is a recipient of a Hildegarde A. Becher Foundation senior fellowship of the Leukemia and Lymphoma Society.",
year = "2003",
month = mar,
day = "1",
doi = "10.1016/S1097-2765(03)00058-3",
language = "English (US)",
volume = "11",
pages = "827--835",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}