Abstract
Glucose-inducible gene expression is a fundamental cellular response for optimal cell growth, but identities of glucose-inducible genes and its regulatory mechanism remain largely elusive in Schizosaccharomyces pombe. Here we report that ura4+, encoding orotidine monophosphate decarboxylase (OMPdecase), shows glucose-inducible expression regulated at post-transcriptional level. The ura4+ mRNA level was rapidly decreased by ∼50% within 20 min after glucose depletion and it was readily recovered upon glucose-readdition within 1 h. Glucose at above 2% similarly raised the transcript level of ura4+, while low concentration (0.1%) was not effective. Interestingly, control of mRNA turnover would be the main regulatory step of the glucose-dependent expression of ura4+. Moreover, stress-activated MAPK (SAPK) pathway was partially responsible for the glucose-regulated expression of ura4+ and rrg1+, another example of glucose-dependent mRNA stability control in S. pombe. These results suggest that the SAPK pathway might participate in the glucose-dependent regulation of ura4+ and rrg1+ mRNA stabilities.
Original language | English (US) |
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Pages (from-to) | 437-443 |
Number of pages | 7 |
Journal | Molecules and Cells |
Volume | 14 |
Issue number | 3 |
DOIs | |
State | Published - Dec 2002 |
Externally published | Yes |
Keywords
- Glucose
- Post-transcriptional Regulation
- Stress-activated MAP Kinase
- mRNA Stability
- rrg1
- ura4
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology