Post-transcriptional regulation of ura4+ gene expression by glucose in Schizosaccharomyces pombe

Min Ji Kim, Minkyu Kim, Sang Dai Park

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Glucose-inducible gene expression is a fundamental cellular response for optimal cell growth, but identities of glucose-inducible genes and its regulatory mechanism remain largely elusive in Schizosaccharomyces pombe. Here we report that ura4+, encoding orotidine monophosphate decarboxylase (OMPdecase), shows glucose-inducible expression regulated at post-transcriptional level. The ura4+ mRNA level was rapidly decreased by ∼50% within 20 min after glucose depletion and it was readily recovered upon glucose-readdition within 1 h. Glucose at above 2% similarly raised the transcript level of ura4+, while low concentration (0.1%) was not effective. Interestingly, control of mRNA turnover would be the main regulatory step of the glucose-dependent expression of ura4+. Moreover, stress-activated MAPK (SAPK) pathway was partially responsible for the glucose-regulated expression of ura4+ and rrg1+, another example of glucose-dependent mRNA stability control in S. pombe. These results suggest that the SAPK pathway might participate in the glucose-dependent regulation of ura4+ and rrg1+ mRNA stabilities.

Original languageEnglish (US)
Pages (from-to)437-443
Number of pages7
JournalMolecules and Cells
Issue number3
StatePublished - Dec 2002
Externally publishedYes


  • Glucose
  • Post-transcriptional Regulation
  • Stress-activated MAP Kinase
  • mRNA Stability
  • rrg1
  • ura4

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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