Possible in vivo 5-HT reuptake blocking properties of 8-OH-DPAT assessed by measuring hippocampal extracellular 5-HT using microdialysis in rats

Marie Bernadette Assié, Wouter Koek

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30 Scopus citations

Abstract

1. The 5-hydroxytryptamine (5-HT)(1A) receptor agonist, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), has been shown to label 5-HT reuptake sites. 2. To study the functional consequences of this property, the effects of 8-OH-DPAT were compared with those of the 5-HT reuptake inhibitors, paroxetine and clomipramine, and of the 5-HT(1A) receptor agonist flesinoxan, in vitro on 5-HT reuptake, and in vivo on the extracellular concentration of 5-HT by use of microdialysis, in rat hippocampus. Because 5-HT reuptake inhibitors reportedly attenuate the ability of (+)-fenfluramine to increase the extracellular concentration of 5-HT, the possible reversal of these effects by 8-OH-DPAT and by paroxetine were examined. 3.. 8-OH-DPAT, paroxetine and clomipramine inhibited [3H]-5-HT reuptake in rat hippocampal synaptosomes (pIC50: 6.00, 8.41 and 7.00, respectively). In contrast, flesinoxan did not alter 5-HT reuptake (pIC50 < 5). 4. 8-OH-DPAT (10 and 100 μM), paroxetine (0.1 μM) and clomipramine (1 μM), administered through the dialysis probe, significantly increased the hippocampal extracellular concentration of 5-HT. In contrast, flesinoxan (100 μM) did not alter extracellular 5-HT. Moreover, the effects of 100 μM 8-OH-DPAT were not blocked by the 5-HT(1A) receptor antagonist, WAY-100635 (0.16 mg kg-1, s.c.). 5. The increase in extracellular 5-HT induced by 10 mg kg-1, i.p., (+)-fenfluramine was prevented not only by 0.1 μM paroxetine, but also by 100 μM 8-OH-DPAT. In addition, systemic administration of 10 mg kg-1, but not 2.5 mg kg-1, i.p. 8-OH-DPAT attenuated the increase in extracellular 5-HT induced by 2.5 mg kg-1, i.p., (+)-fenfluramine. 6. These findings suggest that the increase in extracellular 5-HT produced by local administration of 8-OH-DPAT does not involve its 5-HT(1A) receptor agonist properties, but may result, at least in part, from its 5-HT reuptake blocking properties.

Original languageEnglish (US)
Pages (from-to)845-850
Number of pages6
JournalBritish Journal of Pharmacology
Volume119
Issue number5
DOIs
Publication statusPublished - Jan 1 1996

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Keywords

  • 5-HT reuptake
  • 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT)
  • Extracellular 5-HT concentration
  • Hippocampus
  • Microdialysis

ASJC Scopus subject areas

  • Pharmacology

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