Porcine pancreas extract decreases blood-ionized calcium in mice and inhibits osteoclast formation and bone resorption in culture

Toshiyuki Yoneda, Yoshito Takaoka, Maria M. Alsina, Joyce Garcia, Gregory R. Mundy

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Patients with acute pancreatitis commonly manifest hypocalcemia for reasons which are unknown. We found that porcine pancreas extracts (PX) significantly decreased blood-ionized calcium in Balb/e mice. Partially-purified PX with a molecular mass of approximately 27 kDa decreased blood-ionized calcium in the mice. Partially-purified PX suppressed not only 41Ca release from fetal rat long bones which had been stimulated by parathyroid hormone, interleukin-1 α, tumor necrosis factor, transforming growth factor-α, 1.25-dihydroxyvitamin D3 and prostaglandin E2, but tartrate-resistant acid phosphatase-positive multinucleated cell formation in the presence of 1,25-dihydroxyvitamin D3 in mouse marrow cultures. The results suggest that there is an as yet-unidentified bone metabolism-regulating substance in porcine pancreas which might be responsible for the hypocalcemia associated with acute pancreatitis.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalFEBS Letters
Volume278
Issue number2
DOIs
StatePublished - Jan 28 1991

Keywords

  • Acute pancreatitis
  • Bone resorption
  • Hypocalcemia
  • Osteoclast
  • Porcine pancreas

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Porcine pancreas extract decreases blood-ionized calcium in mice and inhibits osteoclast formation and bone resorption in culture'. Together they form a unique fingerprint.

Cite this