Pooled human immunoglobulins contain anti-idiotypes with reactivity against the SLE-associated 4B4 cross-reactive idiotype

F. DeKeyser, H. DeKeyser, M. D. Kazatchkine, F. Rossi, H. Dang, N. Talal

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Objective: To identify anti-idiotype antibody activity against a regulatory anti-Sm idiotype (4B4) in pooled human IgG (IVIG). Methods: ELISA were used to determine anti-idiotype antibody activity in IVIG. Results: Anti-idiotype antibody activity to 4B4 was detected in IVIG. The anti-idiotypic activity in IVIG could be inhibited by antigen, Sm, and rabbit anti-idiotypic antiserum. The idiotpic binding activity was predominately directed against the 4B4 V(H) region. Conclusion: IVIG contains antibodies against a lupus-associated crossreactive idiotype. These antibodies represent Ab(2β) type antibodies. The anti-idiotypic activity in IVIG may be relevant to the efficacious use of IVIG in treating rheumatic diseases.

Original languageEnglish (US)
Pages (from-to)587-591
Number of pages5
JournalClinical and Experimental Rheumatology
Volume14
Issue number6
StatePublished - Nov 1996

Keywords

  • Sm nucleoprotein
  • idiotype
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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    DeKeyser, F., DeKeyser, H., Kazatchkine, M. D., Rossi, F., Dang, H., & Talal, N. (1996). Pooled human immunoglobulins contain anti-idiotypes with reactivity against the SLE-associated 4B4 cross-reactive idiotype. Clinical and Experimental Rheumatology, 14(6), 587-591.