Abstract
Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.
| Original language | English (US) |
|---|---|
| Journal | Journal of Visualized Experiments |
| Issue number | 87 |
| DOIs | |
| State | Published - May 17 2014 |
| Externally published | Yes |
Keywords
- 4E-BP1
- Biochemistry
- Cell physiological phenomena
- Cells
- Eukaryota
- Genome-wide analysis
- Issue 87
- Metabolic phenomena
- Neoplasms
- Nutritional and metabolic diseases
- Protein synthesis
- Ribosomes
- Translatome
- eIF4E
- mRNA translation
- mTOR
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology
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