Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin, Kristina Sikström, Tommy Alain, Masahiro Morita, Shannon McLaughlan, Ola Larsson, Ivan Topisirovic

Research output: Contribution to journalArticlepeer-review

95 Scopus citations

Abstract

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

Original languageEnglish (US)
JournalJournal of Visualized Experiments
Issue number87
DOIs
StatePublished - May 17 2014
Externally publishedYes

Keywords

  • 4E-BP1
  • Biochemistry
  • Cell physiological phenomena
  • Cells
  • Eukaryota
  • Genome-wide analysis
  • Issue 87
  • Metabolic phenomena
  • Neoplasms
  • Nutritional and metabolic diseases
  • Protein synthesis
  • Ribosomes
  • Translatome
  • eIF4E
  • mRNA translation
  • mTOR

ASJC Scopus subject areas

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint

Dive into the research topics of 'Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale'. Together they form a unique fingerprint.

Cite this