Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin; Kristina Sikström; Tommy Alain; Masahiro Morita; Shannon McLaughlan; Ola Larsson; Ivan Topisirovic

doi:10.3791/51455

Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Valentina Gandin, Kristina Sikström, Tommy Alain, Masahiro Morita, Shannon McLaughlan, Ola Larsson, Ivan Topisirovic

Research output: Contribution to journal › Article › peer-review

162 Scopus citations

Abstract

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

Original language	English (US)
Journal	Journal of Visualized Experiments
Issue number	87
DOIs	https://doi.org/10.3791/51455
State	Published - May 17 2014
Externally published	Yes

Keywords

4E-BP1
Biochemistry
Cell physiological phenomena
Cells
Eukaryota
Genome-wide analysis
Issue 87
Metabolic phenomena
Neoplasms
Nutritional and metabolic diseases
Protein synthesis
Ribosomes
Translatome
eIF4E
mRNA translation
mTOR

ASJC Scopus subject areas

General Chemical Engineering
General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology
General Neuroscience

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10.3791/51455

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abstract = "Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.",

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AU - Gandin, Valentina

AU - Sikström, Kristina

AU - Alain, Tommy

AU - Morita, Masahiro

AU - McLaughlan, Shannon

AU - Larsson, Ola

AU - Topisirovic, Ivan

PY - 2014/5/17

Y1 - 2014/5/17

N2 - Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

AB - Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

KW - 4E-BP1

KW - Biochemistry

KW - Cell physiological phenomena

KW - Cells

KW - Eukaryota

KW - Genome-wide analysis

KW - Issue 87

KW - Metabolic phenomena

KW - Neoplasms

KW - Nutritional and metabolic diseases

KW - Protein synthesis

KW - Ribosomes

KW - Translatome

KW - eIF4E

KW - mRNA translation

KW - mTOR

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JO - Journal of Visualized Experiments

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IS - 87

ER -

Polysome fractionation and analysis of mammalian translatomes on a genome-wide scale

Abstract

Keywords

ASJC Scopus subject areas

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