Polymorphic microsatellite loci for the common marmoset (Callithrix jacchus) designed using a cost- and time-efficient method

M. Raveendran, S. Tardif, C. N. Ross, S. N. Austad, R. A. Harris, A. Milosavljevic, J. Rogers

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We describe a cost- and time-efficient method for designing new microsatellite markers in any species with substantial genomic DNA sequence data available. Using this technique, we report 14 new polymorphic dinucleotide microsatellite loci isolated from the common marmoset. The relative yield of new polymorphisms was higher with less labor than described in previous marmoset studies. Of 20 loci initially evaluated, 14 were polymorphic and amplified reliably (70% success rate). The number of alleles ranged from 3 to 9 with heterozygosity varying from 0.48 to 0.83.

Original languageEnglish (US)
Pages (from-to)906-910
Number of pages5
JournalAmerican Journal of Primatology
Volume70
Issue number9
DOIs
StatePublished - Sep 2008

Keywords

  • Callithrix jacchus
  • Common marmoset
  • Markers
  • Marmoset
  • Microsatellite

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

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