Polymerase chain reaction detection of Aspergillus DNA in experimental models of invasive aspergillosis

Juergen Loeffler, Kerstin Kloepfer, Holger Hebart, Laura Najvar, John R. Graybill, William R. Kirkpatrick, Thomas F. Patterson, Klaus Dietz, Ralf Bialek, Hermann Einsele

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

To determine the sensitivity of polymerase chain reaction (PCR) assays for the diagnosis of invasive aspergillosis, results of quantitative culture, PCR-ELISA, and a quantitative LightCycler assay (Roche Diagnostics) of blood and organ specimens of experimentally infected mice and rabbits were compared. By PCR-ELISA, 297 of 379 murine lung specimens were positive, but only 235 of 379 were culture positive. Whereas 64 culture-negative lungs were positive by PCR, Aspergillus was grown from only 2 PCR-negative samples. The PCR assay was 19.4 times more sensitive than culture. None of the 68 blood cultures from mice and rabbits were positive for Aspergillus fumigatus, whereas PCR detected Aspergillus DNA in 17 of 68 blood samples. Quantitative PCR analysis of blood samples showed a fungus load of 101-102 cfu/mL of blood. The data confirm the superior sensitivity of PCR for the diagnosis of experimental Aspergillus infections.

Original languageEnglish (US)
Pages (from-to)1203-1206
Number of pages4
JournalJournal of Infectious Diseases
Volume185
Issue number8
DOIs
StatePublished - Apr 15 2002

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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