Podoplanin is dispensable for mineralized tissue formation and maintenance in the Swiss outbred mouse background

Masako Toda Nakamura, Honghao Zhang, Dayong Guo, Hiroki Ueharu, Haichun Pan, Greg Scott, Marie Harris, Manas Ray, Jiang Q. Feng, Stephen E. Harris, Lynda F. Bonewald, Yuji Mishina

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Podoplanin, PDPN, is a mucin-type transmembrane glycoprotein widely expressed in many tissues, including lung, kidney, lymph nodes, and mineralized tissues. Its function is critical for lymphatic formation, differentiation of type I alveolar epithelial lung cells, and for bone response to biomechanical loading. It has previously been shown that Pdpn null mice die at birth due to respiratory failure emphasizing the importance of Pdpn in alveolar lung development. During the course of generation of Pdpn mutant mice, we found that most Pdpn null mice in the 129S6 and C57BL6/J mixed genetic background die at the perinatal stage, similar to previously published studies with Pdpn null mice, while all Pdpn null mice bred with Swiss outbred mice survived. Surviving mutant mice in the 129S6 and C57BL6/J mixed genetic background showed alterations in the osteocyte lacunocanalicular network, especially reduced osteocyte canaliculi in the tibial cortex with increased tibial trabecular bone. However, adult Pdpn null mice in the Swiss outbred background showed no overt differences in their osteocyte lacunocnalicular network, bone density, and no overt differences when challenged with exercise. Together, these data suggest that genetic variations present in the Swiss outbred mice compensate for the loss of function of PDPN in lung, kidney, and bone.

Original languageEnglish (US)
Article numbere23450
JournalGenesis
Volume59
Issue number10
DOIs
StatePublished - Oct 2021

Keywords

  • Podoplanin
  • craniofacial development
  • exercise
  • osteocyte
  • skeletogenesis
  • tooth bud

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Podoplanin is dispensable for mineralized tissue formation and maintenance in the Swiss outbred mouse background'. Together they form a unique fingerprint.

Cite this