Pneumococcal gene complex involved in resistance to extracellular oxidative stress

Vahid Farshchi Andisi, Cecilia A. Hinojosa, Anne de Jong, Oscar P. Kuipers, Carlos J. Orihuela, Jetta J.E. Bijlsma

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Streptococcus pneumoniae is a Gram-positive bacterium which is a member of the normal human nasopharyngeal flora but can also cause serious disease such as pneumonia, bacteremia, and meningitis. Throughout its life cycle, S. pneumoniae is exposed to significant oxidative stress derived from endogenously produced hydrogen peroxide (H2O2) and from the host through the oxidative burst. How S. pneumoniae, an aerotolerant anaerobic bacterium that lacks catalase, protects itself against hydrogen peroxide stress is still unclear. Bioinformatic analysis of its genome identified a hypothetical open reading frame belonging to the thiol-specific antioxidant (TlpA/TSA) family, located in an operon consisting of three open reading frames. For all four strains tested, deletion of the gene resulted in an approximately 10-fold reduction in survival when strains were exposed to external peroxide stress. However, no role for this gene in survival of internal superoxide stress was observed. Mutagenesis and complementation analysis demonstrated that all three genes are necessary and sufficient for protection against oxidative stress. Interestingly, in a competitive index mouse pneumonia model, deletion of the operon had no impact shortly after infection but was detrimental during the later stages of disease. Thus, we have identified a gene complex involved in the protection of S. pneumoniae against external oxidative stress, which plays an important role during invasive disease.

Original languageEnglish (US)
Pages (from-to)1037-1049
Number of pages13
JournalInfection and immunity
Volume80
Issue number3
DOIs
StatePublished - Mar 2012

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Pneumococcal gene complex involved in resistance to extracellular oxidative stress'. Together they form a unique fingerprint.

  • Cite this

    Andisi, V. F., Hinojosa, C. A., de Jong, A., Kuipers, O. P., Orihuela, C. J., & Bijlsma, J. J. E. (2012). Pneumococcal gene complex involved in resistance to extracellular oxidative stress. Infection and immunity, 80(3), 1037-1049. https://doi.org/10.1128/IAI.05563-11