Platelet-rich plasma, low-level laser therapy, or their combination promotes periodontal regeneration in fenestration defects: A preliminary in vivo study

Maria J.H. Nagata, Natália De Campos, Michel R. Messora, Natália M. Pola, Carolina S. Santinoni, Suely R.M. Bomfim, Stephen E. Fucini, Edilson Ervolino, Juliano M. De Almeida, Letícia H. Theodoro, Valdir G. Garcia

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Background: This study histomorphometrically analyzes the influence of platelet-rich plasma (PRP), low-level laser therapy (LLLT), or their combination on the healing of periodontal fenestration defects (PFDs) in rats. Methods: PFDs were surgically created in the mandibles of 80 rats. The animals were randomly divided into four groups: 1) C (control) and 2) PRP, defects were filled with blood clot or PRP, respectively; 3) LLLT and 4) PRP/LLLT, defects received laser irradiation, were filled with blood clot or PRP, respectively, and then irradiated again. Animals were euthanized at either 10 or 30 days post-surgery. Percentage of new bone (NB), density of newly formed bone (DNB), new cementum (NC), and extension of remaining defect (ERD) were histomorphometrically evaluated. Data were statistically analyzed (analysis of variance; Tukey test, P <0.05). Results: At 10 days, group PRP presented ERD significantly lower than group C. At 30 days, group PRP presented NB and DNB significantly greater than group C. Groups LLLT, PRP, and PRP/LLLT showed significant NC formation at 30 days, with collagen fibers inserted obliquely or perpendicularly to the root surface. NC formation was not observed in any group C specimen. Conclusions: LLLT, PRP, or their combination all promoted NC formation with a functional periodontal ligament. The combination PRP/LLLT did not show additional positive effects compared to the use of either therapy alone.

Original languageEnglish (US)
Pages (from-to)770-778
Number of pages9
JournalJournal of periodontology
Volume85
Issue number6
DOIs
StatePublished - Jun 2014
Externally publishedYes

Keywords

  • Animal
  • Laser therapy
  • Low-level
  • Models
  • Periodontics
  • Platelet-rich plasma
  • Regeneration
  • Wounds and injuries

ASJC Scopus subject areas

  • Periodontics

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