Platelet-derived microparticles generated by neonatal extracorporeal membrane oxygenation systems

Andrew D. Meyer, Jonathan A.L. Gelfond, Andrew A. Wiles, Robert J. Freishtat, Khoydar Rais-Bahrami

    Research output: Contribution to journalArticlepeer-review

    29 Scopus citations

    Abstract

    Current anticoagulation strategies do not eliminate thromboembolic stroke or limb loss during neonatal extracorporeal membrane oxygenation (ECMO), a form of cardiopulmonary bypass (CPB). In adults, CPB surgery generates prothrombotic platelet-derived microparticles (PMPs), submicron membrane vesicles released from activated platelets. However, information on PMP generation in neonatal ECMO systems is lacking. The objective of this study was to compare PMP generation in five different neonatal ECMO systems, using a simulated circuit with swine blood at 300 ml/min for 4 hours. Systems were composed of both newer components (centrifugal pump and hollow-fiber oxygenator) and traditional components (roller-head pump and silicone membrane oxygenator). Free plasma hemoglobin levels were measured as an indicator of hemolysis and flow cytometry-measured PMP. Hemolysis generated in all ECMO systems was similar to that observed in noncirculated static blood (p = 0.48). There was no difference in net PMP levels between different oxygenators with a given pump. In contrast, net PMP generation in ECMO systems with a centrifugal pump was at least 2.5 times greater than in roller-head pump systems. This was significant when using either a hollow-fiber (p < 0.005) or a silicone membrane (p < 0.05) oxygenator. Future studies are needed to define the relationship between pump-generated PMP and thrombosis.

    Original languageEnglish (US)
    Pages (from-to)37-42
    Number of pages6
    JournalASAIO Journal
    Volume61
    Issue number1
    DOIs
    StatePublished - Jan 13 2015

    Keywords

    • ECMO
    • blood platelets
    • cardiopulmonary bypass
    • cell-derived microparticles
    • flow cytometry
    • hemolysis

    ASJC Scopus subject areas

    • Biophysics
    • Bioengineering
    • Biomaterials
    • Biomedical Engineering

    Fingerprint

    Dive into the research topics of 'Platelet-derived microparticles generated by neonatal extracorporeal membrane oxygenation systems'. Together they form a unique fingerprint.

    Cite this