Platelet-derived growth factor receptor β regulates migration and DNA synthesis in metanephric mesenchymal cells

Mazen Y Arar, Yi Chun Xu, Ihsan Elshihabi, Jeffrey L. Barnes, Goutam Ghosh-choudhury, Hanna E. Abboud

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Platelet-derived growth factor (PDGF) B-chain and PDGF receptor β (PDGFR β) are essential for glomerulogenesis. Mice deficient in PDGF B-chain or PDGFR exhibit an abnormal glomerular phenotype characterized by total lack of mesangial cells. In this study, we localized PDGFR β in the developing rat kidney and explored the biological effects of PDGF in metanephric mesenchymal cells in an attempt to determine the mechanism by which PDGF regulates mesangial cell development. Immunohistochemical and in situ hybridization studies of rat embryonic kidneys reveal that PDGFR β localizes to undifferentiated metanephric mesenchyme and is later expressed in the cleft of the comma-shaped and S-shaped bodies and in more mature glomeruli in a mesangial distribution. We also isolated and characterized cells from rat metanephric mesenchyme. Metanephric mesenchymal cells express vimentin and α-smooth muscle actin but not cytokeratin. These cells also express functional PDGFR β, as demonstrated by autophosphorylation of the receptor as well as activation of phosphatidylinositol 3 kinase in response to PDGF B- chain homodimer. PDGF B-chain also induces migration and proliferation of metanephric mesenchymal cells. Taken together with the fact that PDGF B-chain is expressed in the glomerular epithelium and mesangial area, as demonstrated in the human embryonic kidney, we suggest that PDGF B-chain acts in a paracrine fashion to stimulate the migration and proliferation of mesangial cell precursors from undifferentiated metanephric mesenchyme to the mesangial area. PDGF B-chain also likely stimulates proliferation of mesangial cell precursors in an autocrine fashion once these cells migrate to the glomerular tuft.

Original languageEnglish (US)
Pages (from-to)9527-9533
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number13
DOIs
StatePublished - Mar 31 2000

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Proto-Oncogene Proteins c-sis
Platelet-Derived Growth Factor Receptors
Mesangial Cells
DNA
Mesoderm
Rats
Platelet-Derived Growth Factor
Kidney
Phosphatidylinositol 3-Kinase
Vimentin
Keratins
In Situ Hybridization
Smooth Muscle
Muscle
Actins
Epithelium
Chemical activation
Phenotype

ASJC Scopus subject areas

  • Biochemistry

Cite this

Platelet-derived growth factor receptor β regulates migration and DNA synthesis in metanephric mesenchymal cells. / Arar, Mazen Y; Xu, Yi Chun; Elshihabi, Ihsan; Barnes, Jeffrey L.; Ghosh-choudhury, Goutam; Abboud, Hanna E.

In: Journal of Biological Chemistry, Vol. 275, No. 13, 31.03.2000, p. 9527-9533.

Research output: Contribution to journalArticle

Arar, Mazen Y ; Xu, Yi Chun ; Elshihabi, Ihsan ; Barnes, Jeffrey L. ; Ghosh-choudhury, Goutam ; Abboud, Hanna E. / Platelet-derived growth factor receptor β regulates migration and DNA synthesis in metanephric mesenchymal cells. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 13. pp. 9527-9533.
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