Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis

L. Gesualdo, M. Pinzani, J. J. Floriano, M. O. Hassan, N. U. Nagy, F. P. Schena, S. N. Emancipator, H. E. Abboud

Research output: Contribution to journalArticle

156 Scopus citations

Abstract

Proliferation of mesangial cells and expansion of mesangial matrix are common histologic features of proliferative glomerular diseases, a frequent cause of renal failure. Proliferation of glomerular mesangial cells occurs in response to platelet-derived growth factor (PDGF), and these cells release PDGF and express PDGF A and B chain mRNAs. However, all studies relating PDGF to potential changes in glomerular structure and function to date have been performed in vitro. To explore the role of PDGF in proliferative glomerulonephritides, we studied the expression of PDGF in vivo in two animal models of IgA nephropathy with different histologic patterns of glomerular injury: either predominant mesangial proliferation or expansion of mesangial matrix. Increased expression of PDGF and PDGF B-chain mRNA in whole kidneys from diseased mice was demonstrated by immunohistochemical techniques and by solution hybridization assay, respectively. Immunohistochemically, PDGF was localized primarily within the mesangial area of glomeruli and to a much lower extent in interstitium. The increased PDGF expression correlated with the degree of hypercellularity and clinical features of the disease. In addition, PDGF expression was increased in some forms of human glomerulonephritis, characterized by mesangial proliferation. These findings suggest that PDGF may be a major contributor to mesangial cell proliferation seen in proliferative glomerulonephritides.

Original languageEnglish (US)
Pages (from-to)160-167
Number of pages8
JournalLaboratory Investigation
Volume65
Issue number2
StatePublished - Jan 1 1991

Keywords

  • Cytokines
  • Experimental
  • Growth factors
  • Human
  • Immune complex disease
  • Kidney

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis'. Together they form a unique fingerprint.

  • Cite this

    Gesualdo, L., Pinzani, M., Floriano, J. J., Hassan, M. O., Nagy, N. U., Schena, F. P., Emancipator, S. N., & Abboud, H. E. (1991). Platelet-derived growth factor expression in mesangial proliferative glomerulonephritis. Laboratory Investigation, 65(2), 160-167.