Platelet activating factor (PAF) induced release of platelet factor 4 (PF4) in vitro and during IgE anaphylaxis in the rabbit

Linda M Mcmanus, C. A. Morley, S. P. Levin, R. N Pinckard

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Abstract

Basophil-derived platelet activating factor (PAF) was shown to induce the release of platelet factor 4 (PF4), a specific plaelet protein, from isolated, washed rabbit platelets. The kinetics and dose response of this release were similar to that of PA-induced 3H-serotonin secretion, and indicated that PF4 release was a reliable and sensitive estimate of platelet stimulation y PAF. Plasma PF4 concentrations were therefore used as a marker for in vivo platelet release during systemic anaphylaxis inducd by the i.v. injection of antigen (horseradish peroxidase, HRP) into rabbits conditioned to produce only IgE antibody against RP. Antigen administration was accompanied by the loss of circulating stainable basophils, acute thrombocytopenia and neutropena, and a significant increase in PF4 levels. The increase in plasma PF4 began approximately 90 sec after antigen challenge, coresponding to 60 sec after the intravascular aggregation of platelets. However, maximum levels of plasma PF4 were not reached unil 3 to 5 min after antigen challenge. Concomitant measurement of circulating PAF during anaphylaxis indicated that PAF releaseinto the circulation preceded the decrease in circulating platelet numbers by about 30 sec and preceded the increase in plasma PF4 levels by about 60 sec. Thus, this study documents that platelets undergo a release reaction during anaphylaxis at a time hen they are sequestered in the microvasculature. The temporal relationship of this release suggests that the following series f events occur during IgE anaphylaxis: antigen stimulates circulating IgE-sensitized basophils to release PAF, PAF causes intraascular platelet aggregation and subsequent sequestration, and finally, sequestered platelets release their intracellular consttuents.

Original languageEnglish (US)
Pages (from-to)2835-2842
Number of pages8
JournalJournal of Immunology
Volume123
Issue number6
StatePublished - 1979

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Platelet Factor 4
Platelet Activating Factor
Anaphylaxis
Immunoglobulin E
Rabbits
Blood Platelets
Basophils
Antigens
Platelet Aggregation
Horseradish Peroxidase
Microvessels
In Vitro Techniques
Platelet Count
Thrombocytopenia
Serotonin
Injections
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Platelet activating factor (PAF) induced release of platelet factor 4 (PF4) in vitro and during IgE anaphylaxis in the rabbit. / Mcmanus, Linda M; Morley, C. A.; Levin, S. P.; Pinckard, R. N.

In: Journal of Immunology, Vol. 123, No. 6, 1979, p. 2835-2842.

Research output: Contribution to journalArticle

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