Platelet activating factor and lyso-phosphatidylcholines from strawberry

Mary Calligerou; Athanassia Siafaka-Kapadai; Dia Galanopoulou; Suzan T. Weintraub; Mary Mavri-Vavayanni

doi:10.1016/0031-9422(95)00605-2

Platelet activating factor and lyso-phosphatidylcholines from strawberry

Mary Calligerou, Athanassia Siafaka-Kapadai, Dia Galanopoulou, Suzan T. Weintraub, Mary Mavri-Vavayanni

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Lipids from strawberry fruits, leaves, achenes and pollen were separated into classes by TLC, purified by HPLC and tested for biological activity. A lipid fraction from fruits with the same chromatographic behaviour as authentic platelet activating factor (PAF) showed identical biological activity, namely, dose-dependent aggregation of washed rabbit platelets, inhibition of aggregation by CV 3988, platelet desensitization to PAF and vice versa, and loss of activity by alkaline hydrolysis and recovery of activity by reacetylation. The presence of PAF was confirmed by FAB mass spectrometry. Lyso-phosphatidylcholines, including lyso-PAF, were also found in all the plant parts tested.

Original language	English (US)
Pages (from-to)	89-92
Number of pages	4
Journal	Phytochemistry
Volume	41
Issue number	1
DOIs	https://doi.org/10.1016/0031-9422(95)00605-2
State	Published - Jan 1 1996
Externally published	Yes

Keywords

Ether lipids
Fragaria ananassa
Lyso-PAF
Phospholipids
Platelet activating factor (PAF)
Rosaceae
Strawberry

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/0031-9422(95)00605-2

Cite this

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title = "Platelet activating factor and lyso-phosphatidylcholines from strawberry",

abstract = "Lipids from strawberry fruits, leaves, achenes and pollen were separated into classes by TLC, purified by HPLC and tested for biological activity. A lipid fraction from fruits with the same chromatographic behaviour as authentic platelet activating factor (PAF) showed identical biological activity, namely, dose-dependent aggregation of washed rabbit platelets, inhibition of aggregation by CV 3988, platelet desensitization to PAF and vice versa, and loss of activity by alkaline hydrolysis and recovery of activity by reacetylation. The presence of PAF was confirmed by FAB mass spectrometry. Lyso-phosphatidylcholines, including lyso-PAF, were also found in all the plant parts tested.",

keywords = "Ether lipids, Fragaria ananassa, Lyso-PAF, Phospholipids, Platelet activating factor (PAF), Rosaceae, Strawberry",

author = "Mary Calligerou and Athanassia Siafaka-Kapadai and Dia Galanopoulou and Weintraub, {Suzan T.} and Mary Mavri-Vavayanni",

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AU - Calligerou, Mary

AU - Siafaka-Kapadai, Athanassia

AU - Galanopoulou, Dia

AU - Weintraub, Suzan T.

AU - Mavri-Vavayanni, Mary

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Lipids from strawberry fruits, leaves, achenes and pollen were separated into classes by TLC, purified by HPLC and tested for biological activity. A lipid fraction from fruits with the same chromatographic behaviour as authentic platelet activating factor (PAF) showed identical biological activity, namely, dose-dependent aggregation of washed rabbit platelets, inhibition of aggregation by CV 3988, platelet desensitization to PAF and vice versa, and loss of activity by alkaline hydrolysis and recovery of activity by reacetylation. The presence of PAF was confirmed by FAB mass spectrometry. Lyso-phosphatidylcholines, including lyso-PAF, were also found in all the plant parts tested.

AB - Lipids from strawberry fruits, leaves, achenes and pollen were separated into classes by TLC, purified by HPLC and tested for biological activity. A lipid fraction from fruits with the same chromatographic behaviour as authentic platelet activating factor (PAF) showed identical biological activity, namely, dose-dependent aggregation of washed rabbit platelets, inhibition of aggregation by CV 3988, platelet desensitization to PAF and vice versa, and loss of activity by alkaline hydrolysis and recovery of activity by reacetylation. The presence of PAF was confirmed by FAB mass spectrometry. Lyso-phosphatidylcholines, including lyso-PAF, were also found in all the plant parts tested.

KW - Ether lipids

KW - Fragaria ananassa

KW - Lyso-PAF

KW - Phospholipids

KW - Platelet activating factor (PAF)

KW - Rosaceae

KW - Strawberry

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Platelet activating factor and lyso-phosphatidylcholines from strawberry

Abstract

Keywords

ASJC Scopus subject areas

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