@article{5c00619dce764387813212fa82db9154,
title = "Plasmodium falciparum genetic crosses in a humanized mouse model",
abstract = "Genetic crosses of phenotypically distinct strains of the human malaria parasite Plasmodium falciparum are a powerful tool for identifying genes controlling drug resistance and other key phenotypes. Previous studies relied on the isolation of recombinant parasites from splenectomized chimpanzees, a research avenue that is no longer available. Here we demonstrate that human-liver chimeric mice support recovery of recombinant progeny for the identification of genetic determinants of parasite traits and adaptations.",
author = "Vaughan, {Ashley M.} and Pinapati, {Richard S.} and Cheeseman, {Ian H.} and Nelly Camargo and Matthew Fishbaugher and Checkley, {Lisa A.} and Shalini Nair and Hutyra, {Carolyn A.} and Nosten, {Fran{\c c}ois H.} and Anderson, {Timothy J.C.} and Ferdig, {Michael T.} and Kappe, {Stefan H.I.}",
note = "Funding Information: We thank the Center for Infectious Disease Research (formerly Seattle BioMed) insectary and vivarium for mosquito and rodent care, respectively, as well as R. Garcia and M. McDew-White at the Texas Biomedical Research Institute for technical assistance. We thank S. Mikolajczak, E. Wilson and J. Bial for ongoing FRG huHep mouse discussions and M. Macarulay and K. Ushimaru for help with parasite cloning. Thanks also to A. Kaushansky for help with graphics. NIH grants R21 AI 115194–01 to A.M.V. and M.T.F., R37 AI 048071 to T.J.C.A. and Chemistry-Biochemistry-Biology Interface Training Fellowship T32 GM075762 to R.S.P. supported this work, as did Seattle BioMed internal funds to S.H.I.K. The AT&T Genomics Computing Center at Texas Biomedical Research Institute is supported by the AT&T Foundation and the US National Center for Research Resources (NCRR) grant number S10 RR029392, and laboratory work was conducted in facilities constructed with support from Research Facilities Improvement Program grant C06 RR013556 and RR017515 from NCRR.",
year = "2015",
month = jun,
day = "30",
doi = "10.1038/nmeth.3432",
language = "English (US)",
volume = "12",
pages = "631--633",
journal = "Nature Methods",
issn = "1549-1277",
publisher = "Public Library of Science",
number = "7",
}