Plasma symmetric dimethylarginine reference limits from the Framingham offspring cohort

Edzard Schwedhelm, Vanessa Xanthakis, Renke Maas, Lisa M. Sullivan, Dorothee Atzler, Nicole Lüneburg, Nicole L. Glazer, Ulrich Riederer, Ramachandran S. Vasan, Rainer H. Böger

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Background: Symmetric dimethylarginine (SDMA) is a by-product of protein methylation. Once released from proteins, SDMA is eliminated by the kidneys; consequently, plasma concentration has been suggested as a sensitive marker of renal function. Furthermore, recent work implicates SDMA in the pathogenesis of cardiovascular disease. To date, reference limits for SDMA plasma concentrations in healthy individuals are lacking. Methods: This study defined reference limits for plasma SDMA concentrations in 840 relatively healthy individuals of the Offspring Cohort from Framingham Heart Study (mean age 56 years, 61% women). Plasma SDMA concentrations were determined by LC-MS/MS using a stable isotope dilution assay. Results: The median SDMA concentration in the reference sample was 0.37 μmol/L (Q1, Q3:0.32, 0.43 μmol/L) and the reference limits were 0.225 and 0.533 (2.5th and 97.5th percentile). In a multivariable regression model, serum creatinine, age and total homocysteine were positively associated with SDMA (p<0.001 for all), whereas the body mass index and diastolic blood pressure were inversely related to SDMA (p-values<0.01 and 0.03, respectively). Conclusions: This study reports plasma SDMA reference limits from the community-based Framingham Heart Study. Plasma SDMA concentration was related positively to advancing age, but inversely to renal function. These reference limits may allow the identification of individuals with raised plasma SDMA concentrations.

Original languageEnglish (US)
Pages (from-to)1907-1910
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Issue number11
StatePublished - Nov 1 2011
Externally publishedYes


  • Framingham Heart Study
  • LC-MS/MS
  • Symmetric dimethylarginine

ASJC Scopus subject areas

  • Biochemistry, medical
  • Clinical Biochemistry


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