Plasma protein and apolipoprotein synthesis by human yolk sac carcinoma cells in vitro

R. E. Lanford, D. L. Bronson, L. E. Estlack, F. H. Wians

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Three human yolk sac carcinoma cell lines were characterized for the expression of several markers. Each of the cell lines expressed alpha-fetoprotein, without detectable levels of chorionic gonadotropin, and the level of alpha-fetoprotein expression increased dramatically when the cultures were held without passage for extended periods. The secretion of a number of plasma proteins was documented by metabolic labeling, immunoprecipitation, and gel analysis. The major plasma proteins detected were alpha-1-antitrypsin, alpha-fetoprotein, transthyretin, β-2 microglobulin, and plasminogen, with lower levels of transferrin and complement C4 released. Apolipoproteins B, E, and A1 were secreted in high levels as well and were found in the form of lipoprotein particles. Time course experiments on the synthesis of apolipoproteins E and A1 indicated that, as with alpha-fetoprotein, the level of synthesis increased substantially when the cultures were held without passage. The results indicate that these yolk sac carcinoma cells display a protein expression profile similar to that observed for the human yolk sac, and the possibility that the cells may have the potential to differentiate is discussed.

Original languageEnglish (US)
Pages (from-to)205-210
Number of pages6
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume27 I
Issue number3
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology


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