Plasma protease inhibitor (PI) system in the laboratory opossum, Monodelphis domestica

Helen Arthur; Kevin Bell; John L. VandeBerg; Roland A.H. Van Oorschot

doi:10.1007/BF00554414

Plasma protease inhibitor (PI) system in the laboratory opossum, Monodelphis domestica

Helen Arthur, Kevin Bell, John L. VandeBerg, Roland A.H. Van Oorschot

Research output: Contribution to journal › Article › peer-review

Abstract

Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α₁-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PI(D), PI(E), PI(F), PI(G), PI(H), PI(I), PI(J), PI(K), and PI(M), and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.

Original language	English (US)
Pages (from-to)	389-399
Number of pages	11
Journal	Biochemical Genetics
Volume	34
Issue number	9-10
DOIs	https://doi.org/10.1007/BF00554414
State	Published - Dec 1 1996

Keywords

Monodelphis domestica
genetics
polymorphism
protease inhibitor

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Biochemistry
Molecular Biology
Genetics

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10.1007/BF00554414

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title = "Plasma protease inhibitor (PI) system in the laboratory opossum, Monodelphis domestica",

abstract = "Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PI(D), PI(E), PI(F), PI(G), PI(H), PI(I), PI(J), PI(K), and PI(M), and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.",

keywords = "Monodelphis domestica, genetics, polymorphism, protease inhibitor",

author = "Helen Arthur and Kevin Bell and VandeBerg, {John L.} and {Van Oorschot}, {Roland A.H.}",

year = "1996",

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doi = "10.1007/BF00554414",

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AU - Arthur, Helen

AU - Bell, Kevin

AU - VandeBerg, John L.

AU - Van Oorschot, Roland A.H.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PI(D), PI(E), PI(F), PI(G), PI(H), PI(I), PI(J), PI(K), and PI(M), and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.

AB - Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PI(D), PI(E), PI(F), PI(G), PI(H), PI(I), PI(J), PI(K), and PI(M), and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.

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