Plasma protease inhibitor (PI) system in the laboratory opossum, Monodelphis domestica

Helen Arthur, Kevin Bell, John L. VandeBerg, Roland A.H. Van Oorschot

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Protease inhibitor (PI) polymorphism was observed in the laboratory opossum, Monodelphis domestica, by either one-dimensional acid polyacrylamide gel electrophoresis (PAGE; pH 4.6) or isoelectric focusing (pH 3.5-5.0) followed by immunoblotting with rabbit antiserum to human α1-antitrypsin; but acid PAGE produced superior resolution of the PI proteins. Family studies demonstrated an inheritance of nine codominant autosomal alleles, PI(D), PI(E), PI(F), PI(G), PI(H), PI(I), PI(J), PI(K), and PI(M), and a population study revealed frequencies of 0.411, 0.010, 0.341, 0.034, 0.023, 0.071, 0.035, 0.020, and 0.055, respectively.

    Original languageEnglish (US)
    Pages (from-to)389-399
    Number of pages11
    JournalBiochemical Genetics
    Volume34
    Issue number9-10
    DOIs
    StatePublished - Dec 1 1996

    Keywords

    • Monodelphis domestica
    • genetics
    • polymorphism
    • protease inhibitor

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Biochemistry
    • Molecular Biology
    • Genetics

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