Plasma membrane localization of TRPC channels: Role of caveolar lipid rafts

Indu S. Ambudkar, So Ching Brazer, Xibao Liu, Timothy Lockwich, Brij B Singh

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

GPCR-mediated activation of the Ca2+ signalling cascade leads to stimulation of Ca2+ in£ux into non-excitable cells. Both store-dependent and independent channels likely contribute towards this Ca2+ in£ux. However, the identity of the channels and exact mechanism by which they are activated remains elusive. The TRPC family of proteins has been proposed as molecular components of these channels. Studies from our laboratory and others have shown that mammalian TRPC proteins are assembled in a multiprotein complex that includes various key Ca2+ signalling proteins. However, relatively little is known regarding the mechanisms involved in the assembly of the TRPC channel complex in the plasma membrane. We have reported that TRPC1 and TRPC3 signalling complexes are associated with caveolar lipid raft domains (LRDs) in the plasma membrane. Recently we have examined the role of caveolin-1 in the regulation of TRPC channels and store-operated Ca2+ entry (SOCE). Based on our studies, we suggest that (1) caveolin 1 has a potentially critical role in the localization of TRPC channels plasma membrane caveolar LRDs, and (2) the molecular architecture of caveolae can facilitate intramolecular interactions between TRPC channels and associated proteins that are involved in activation and/or inactivation of SOCE.

Original languageEnglish (US)
Title of host publicationMammalian TRP Channels as Molecular Targets
Publisherwiley
Pages63-74
Number of pages12
ISBN (Electronic)9780470862582
ISBN (Print)9780470862544
DOIs
StatePublished - Oct 7 2008
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Ambudkar, I. S., Brazer, S. C., Liu, X., Lockwich, T., & Singh, B. B. (2008). Plasma membrane localization of TRPC channels: Role of caveolar lipid rafts. In Mammalian TRP Channels as Molecular Targets (pp. 63-74). wiley. https://doi.org/10.1002/0470862580