PKC, p42/p44 MAPK, and p38 MAPK are required for HGF-induced proliferation of H441 cells

Vibhudutta Awasthi, Richard J. King

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

In this paper, we studied the signaling pathway used by hepatocyte growth factor/scatter factor (HGF) to stimulate mitosis. We show, using H441 cells, that 1) HGF activates membrane-associated protein kinase C (PKC); the activity is transient and peaks within 30 min; 2) HGF activates p42/p44 and p38 mitogen-activated protein kinases (MAPKs); maximum activity in both is within 10 min; and 3) the activation of neither p38 nor p42/p44 MAPK is dependent on PKC, indicating that HGF uses separate and nonintersecting pathways to activate these two classes of kinase. However, phorbol 12-myristate 13-acetate also activates both MAPKs as well as PKC, but this activation is abolished in cells pretreated with the PKC inhibitor GF-109203X. HGF was found to significantly increase [3H]thymidine incorporation within 5 h; peak thymidine incorporation was observed at 16 h. However, when cells were pretreated with inhibitors of p42/p44 (PD-98059), p38 (SB-203580), or PKC (GF-109203X, Go-6983, or myristoylated inhibitor peptide19-27), HGF-induced thymidine uptake was diminished in a dose-dependent manner. Taken together, these results demonstrate that HGF activates PKC and both MAPKs simultaneously through parallel pathways and that the activation of the MAPKs does not depend on PKC. However, p38 and p42/p44 MAPKs and PKC may all be essential for HGF-induced proliferation of H441 cells.

Original languageEnglish (US)
Pages (from-to)L942-L949
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume279
Issue number5 23-5
DOIs
StatePublished - 2000

Keywords

  • Extracellular signal-regulated kinase
  • Hepatocyte growth factor
  • Lung injury
  • Mitogen-activated protein kinase
  • Protein kinase C

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Fingerprint

Dive into the research topics of 'PKC, p42/p44 MAPK, and p38 MAPK are required for HGF-induced proliferation of H441 cells'. Together they form a unique fingerprint.

Cite this