TY - JOUR
T1 - Pixel-by-Pixel Spatiotemporal Progression of Focal Ischemia Derived Using Quantitative Perfusion and Diffusion Imaging
AU - Shen, Qiang
AU - Meng, Xiangjun
AU - Fisher, Marc
AU - Sotak, Christopher H.
AU - Duong, Timothy Q.
PY - 2003/12
Y1 - 2003/12
N2 - Pixel-by-pixel spatiotemporal progression of focal ischemia (permanent occlusion) in rats was investigated using quantitative perfusion and diffusion magnetic resonance imaging every 30 minutes for 3 hours. The normal left-hemisphere apparent diffusion coefficient (ADC) was 0.76 ± 0.03 × 10-3 mm2/s and CBF was 0.7 ± 0.3 mL · g-1 · min-1 (mean ± SD, n=5). The ADC and CBF viability thresholds yielding the lesion volumes (LV) at 3 hours that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes (200 ± 30 mm3) at 24 hours were 0.53 ± 0.02 × 10-3 mm2/s (30% ± 2% reduction) and 0.30 ± 0.09 mL × g-1 · min-1 (57% ± 11% reduction), respectively. Temporal evolution of the ADC- and CBF-defined LV showed a significant "perfusion-diffusion mismatch" up to 2 hours (P < 0.05, n = 11), a potential therapeutic window. Based on the viability thresholds, three pixel clusters were identified on the CBF-ADC scatterplots: (1) a "normal" cluster with normal CBF and ADC, (2) an "ischemic core" cluster with markedly reduced CBF and ADC, and (3) a "mismatch" cluster with reduced CBF but slightly reduced ADC. These clusters were color-coded and mapped onto the image and CBF-ADC spaces. Lesions grew peripheral and medial to the initial ADC abnormality. In contrast to the CBF distribution, the ADC distribution in the ischemic hemisphere was bimodal; the relatively time-invariant bimodal-ADC minima were 0.57 ± 0.02 × 10-3 mm2/s (corresponding CBF 0.35 ± 0.04 mL · g-1 · min-1, surprisingly similar to the TTC-derived thresholds. Together, these results illustrate an analysis approach to systemically track the pixel-by-pixel spatiotemporal progression of acute ischemic brain injury.
AB - Pixel-by-pixel spatiotemporal progression of focal ischemia (permanent occlusion) in rats was investigated using quantitative perfusion and diffusion magnetic resonance imaging every 30 minutes for 3 hours. The normal left-hemisphere apparent diffusion coefficient (ADC) was 0.76 ± 0.03 × 10-3 mm2/s and CBF was 0.7 ± 0.3 mL · g-1 · min-1 (mean ± SD, n=5). The ADC and CBF viability thresholds yielding the lesion volumes (LV) at 3 hours that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes (200 ± 30 mm3) at 24 hours were 0.53 ± 0.02 × 10-3 mm2/s (30% ± 2% reduction) and 0.30 ± 0.09 mL × g-1 · min-1 (57% ± 11% reduction), respectively. Temporal evolution of the ADC- and CBF-defined LV showed a significant "perfusion-diffusion mismatch" up to 2 hours (P < 0.05, n = 11), a potential therapeutic window. Based on the viability thresholds, three pixel clusters were identified on the CBF-ADC scatterplots: (1) a "normal" cluster with normal CBF and ADC, (2) an "ischemic core" cluster with markedly reduced CBF and ADC, and (3) a "mismatch" cluster with reduced CBF but slightly reduced ADC. These clusters were color-coded and mapped onto the image and CBF-ADC spaces. Lesions grew peripheral and medial to the initial ADC abnormality. In contrast to the CBF distribution, the ADC distribution in the ischemic hemisphere was bimodal; the relatively time-invariant bimodal-ADC minima were 0.57 ± 0.02 × 10-3 mm2/s (corresponding CBF 0.35 ± 0.04 mL · g-1 · min-1, surprisingly similar to the TTC-derived thresholds. Together, these results illustrate an analysis approach to systemically track the pixel-by-pixel spatiotemporal progression of acute ischemic brain injury.
KW - DWI
KW - Oligemia
KW - PWI
KW - Penumbra
KW - Perfusion-diffusion mismatch
KW - Stroke
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U2 - 10.1097/01.WCB.0000100064.36077.03
DO - 10.1097/01.WCB.0000100064.36077.03
M3 - Article
C2 - 14663344
AN - SCOPUS:0346881348
SN - 0271-678X
VL - 23
SP - 1479
EP - 1488
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
IS - 12
ER -