Pixel-by-Pixel Spatiotemporal Progression of Focal Ischemia Derived Using Quantitative Perfusion and Diffusion Imaging

Qiang Shen, Xiangjun Meng, Marc Fisher, Christopher H. Sotak, Timothy Q. Duong

Research output: Contribution to journalArticlepeer-review

105 Scopus citations

Abstract

Pixel-by-pixel spatiotemporal progression of focal ischemia (permanent occlusion) in rats was investigated using quantitative perfusion and diffusion magnetic resonance imaging every 30 minutes for 3 hours. The normal left-hemisphere apparent diffusion coefficient (ADC) was 0.76 ± 0.03 × 10-3 mm2/s and CBF was 0.7 ± 0.3 mL · g-1 · min-1 (mean ± SD, n=5). The ADC and CBF viability thresholds yielding the lesion volumes (LV) at 3 hours that best approximated the 2,3,5-triphenyltetrazolium chloride (TTC) infarct volumes (200 ± 30 mm3) at 24 hours were 0.53 ± 0.02 × 10-3 mm2/s (30% ± 2% reduction) and 0.30 ± 0.09 mL × g-1 · min-1 (57% ± 11% reduction), respectively. Temporal evolution of the ADC- and CBF-defined LV showed a significant "perfusion-diffusion mismatch" up to 2 hours (P < 0.05, n = 11), a potential therapeutic window. Based on the viability thresholds, three pixel clusters were identified on the CBF-ADC scatterplots: (1) a "normal" cluster with normal CBF and ADC, (2) an "ischemic core" cluster with markedly reduced CBF and ADC, and (3) a "mismatch" cluster with reduced CBF but slightly reduced ADC. These clusters were color-coded and mapped onto the image and CBF-ADC spaces. Lesions grew peripheral and medial to the initial ADC abnormality. In contrast to the CBF distribution, the ADC distribution in the ischemic hemisphere was bimodal; the relatively time-invariant bimodal-ADC minima were 0.57 ± 0.02 × 10-3 mm2/s (corresponding CBF 0.35 ± 0.04 mL · g-1 · min-1, surprisingly similar to the TTC-derived thresholds. Together, these results illustrate an analysis approach to systemically track the pixel-by-pixel spatiotemporal progression of acute ischemic brain injury.

Original languageEnglish (US)
Pages (from-to)1479-1488
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume23
Issue number12
DOIs
StatePublished - Dec 2003

Keywords

  • DWI
  • Oligemia
  • PWI
  • Penumbra
  • Perfusion-diffusion mismatch
  • Stroke

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

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