Pinoline and melatonin protect against H2O2-induced lipid peroxidation in rat brain homogenates

Tina J.P. Frederiksen, Gitte Pless, Joaquin J. Garcia, Russel J. Reiter

Research output: Contribution to journalArticlepeer-review


The brain is particularly susceptible to free radical damage, and it is therefore important to find agents that protect against this process. The chief secretory product of the pineal gland, melatonin, is a well known antioxidant. Pinoline is a recently discovered compound with structural similarity to melatonin. In the search for a new neuroprotective agent, the antioxidant capacity of pinoline is compared to that of melatonin. Lipid peroxidation was induced by adding H2O2 to rat brain homogenates. The degree of lipid peroxidation was assessed by estimating the levels of thiobarbituric acid reactive substances, malondialdehyde (MDA) and 4-hydroxyalkenals (4-HDA). The antioxidant capacity of pinoline and melatonin was measured as percent inhibition of lipid peroxidation in rat brain homogenates after the addition of H2O2. Both pinoline and melatonin were found to inhibit lipid peroxidation in a dose-dependent manner. Under these in vitro conditions, pinoline was found to be 7 times more effective than melatonin in reducing MDA + 4-HDA levels, with an IC50 = 0.1 mM for pinoline and an IC50 = 0.7 mM for melatonin.

Original languageEnglish (US)
Pages (from-to)117-123
Number of pages7
JournalNeuroendocrinology Letters
Issue number3
StatePublished - 1998

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Clinical Neurology
  • Psychiatry and Mental health


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