Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine

Juan C. Sáez, Viviana M. Berthoud, Ranjana Kadle, Otto Traub, Bruce J. Nicholson, Michael V.L. Bennett, Rolf Dermietzel

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalBrain Research
Volume568
Issue number1-2
DOIs
StatePublished - Dec 24 1991
Externally publishedYes

Keywords

  • Connexin
  • Coupling
  • Gap junction
  • Pinealocyte
  • astrocyte
  • norepinephrine

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine'. Together they form a unique fingerprint.

  • Cite this

    Sáez, J. C., Berthoud, V. M., Kadle, R., Traub, O., Nicholson, B. J., Bennett, M. V. L., & Dermietzel, R. (1991). Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine. Brain Research, 568(1-2), 265-275. https://doi.org/10.1016/0006-8993(91)91407-R