Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine

Juan C. Sáez; Viviana M. Berthoud; Ranjana Kadle; Otto Traub; Bruce J. Nicholson; Michael V.L. Bennett; Rolf Dermietzel

doi:10.1016/0006-8993(91)91407-R

Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine

Juan C. Sáez, Viviana M. Berthoud, Ranjana Kadle, Otto Traub, Bruce J. Nicholson, Michael V.L. Bennett, Rolf Dermietzel

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

Original language	English (US)
Pages (from-to)	265-275
Number of pages	11
Journal	Brain Research
Volume	568
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(91)91407-R
State	Published - Dec 24 1991
Externally published	Yes

Keywords

Connexin
Coupling
Gap junction
Pinealocyte
astrocyte
norepinephrine

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(91)91407-R

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title = "Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine",

abstract = "Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.",

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author = "S{\'a}ez, {Juan C.} and Berthoud, {Viviana M.} and Ranjana Kadle and Otto Traub and Nicholson, {Bruce J.} and Bennett, {Michael V.L.} and Rolf Dermietzel",

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doi = "10.1016/0006-8993(91)91407-R",

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T1 - Pinealocytes in rats

T2 - connexin identification and increase in coupling caused by norepinephrine

AU - Sáez, Juan C.

AU - Berthoud, Viviana M.

AU - Kadle, Ranjana

AU - Traub, Otto

AU - Nicholson, Bruce J.

AU - Bennett, Michael V.L.

AU - Dermietzel, Rolf

PY - 1991/12/24

Y1 - 1991/12/24

N2 - Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

AB - Dye coupling was observed between pinealocytes in acutely dissected pineal glands of adult rats. Pinealocytes maintained in culture were also electrically coupled. Connexins 26 and 43 and their respective mRNAs were present but neither connexin32 nor itsmRNA were detected. Pinealocytes expressed only connexin26 whereas connexin43 was confined to astrocytes. In 5-day-old cultures of pinealocytes the incidence of dye coupling and level of immunodetectable connexin26 were low, and both were increased by norepinephrine (NE). The increase in incidence of coupling was maximal at around 6 h after treatment and was prevented by inhibitors of protein of mRNA synthesis. NE-induced metabolic and electrical synchronization mediated by gap junctions may favor melatonin secretion.

KW - Connexin

KW - Coupling

KW - Gap junction

KW - Pinealocyte

KW - astrocyte

KW - norepinephrine

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JO - Brain Research

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Pinealocytes in rats: connexin identification and increase in coupling caused by norepinephrine

Abstract

Keywords

ASJC Scopus subject areas

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