Pih1p-Tah1p Puts a Lid on Hexameric AAA+ ATPases Rvb1/2p

Shaoxiong Tian, Ge Yu, Huan He, Yu Zhao, Peilu Liu, Alan G. Marshall, Borries Demeler, Scott M. Stagg, Hong Li

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The Saccharomyces cerevisiae (Sc) R2TP complex affords an Hsp90-mediated and nucleotide-driven chaperone activity to proteins of small ribonucleoprotein particles (snoRNPs). The current lack of structural information on the ScR2TP complex, however, prevents a mechanistic understanding of this biological process. We characterized the structure of the ScR2TP complex made up of two AAA+ ATPases, Rvb1/2p, and two Hsp90 binding proteins, Tah1p and Pih1p, and its interaction with the snoRNP protein Nop58p by a combination of analytical ultracentrifugation, isothermal titration calorimetry, chemical crosslinking, hydrogen-deuterium exchange, and cryoelectron microscopy methods. We find that Pih1p-Tah1p interacts with Rvb1/2p cooperatively through the nucleotide-sensitive domain of Rvb1/2p. Nop58p further binds Pih1p-Tahp1 on top of the dome-shaped R2TP. Consequently, nucleotide binding releases Pih1p-Tah1p from Rvb1/2p, which offers a mechanism for nucleotide-driven binding and release of snoRNP intermediates. Tian et al. report that AAA+ ATPases, Rvb1/2p, though previously shown to form both dodecamer and hexamer, form a hexameric base for binding co-chaperone Pih1p-Tah1p. Tian et al. also showed that the binding interface is made of flexible and nucleotide-sensitive insertions within Rvb1/2p, which offers the basis for nucleotide-driven client release.

Original languageEnglish (US)
Pages (from-to)1519-1529.e4
JournalStructure
Volume25
Issue number10
DOIs
StatePublished - Oct 3 2017

Keywords

  • AAA+ proteins
  • FT-ICR
  • FTMS
  • Fourier transform mass spectrometry
  • Rvb1
  • Rvb2
  • analytical ultracentrifugation
  • cryoelectron microscopy
  • hydrogen/deuterium exchange
  • ion cyclotron resonance

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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