Pigmentation in NIR Laser Tissue Damage

Michael L. Denton; Debbie M. Eikum; Gary D. Noojin; David J. Stolarski; Robert J. Thomas; Randolph D. Glickman; Benjamin A. Rockwell

doi:10.1117/12.476898

Pigmentation in NIR Laser Tissue Damage

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Robert J. Thomas, Randolph D. Glickman, Benjamin A. Rockwell

Ophthalmology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of MR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	B.E. Stuck, M Belkin
Pages	78-84
Number of pages	7
Volume	4953
DOIs	https://doi.org/10.1117/12.476898
State	Published - 2003
Event	Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III - San Jose,CA, United States Duration: Jan 26 2003 → Jan 27 2003

Other

Other	Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III
Country	United States
City	San Jose,CA
Period	1/26/03 → 1/27/03

Keywords

Artificial pigmentation
Cytotoxicity threshold
Fluorescence microscopy
hTERT-RPE1 cells
Mode-locked

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

Access to Document

10.1117/12.476898

Fingerprint Dive into the research topics of 'Pigmentation in NIR Laser Tissue Damage'. Together they form a unique fingerprint.

Cite this

Denton, M. L., Eikum, D. M., Noojin, G. D., Stolarski, D. J., Thomas, R. J., Glickman, R. D., & Rockwell, B. A. (2003). Pigmentation in NIR Laser Tissue Damage. In B. E. Stuck, & M. Belkin (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4953, pp. 78-84) https://doi.org/10.1117/12.476898

Pigmentation in NIR Laser Tissue Damage. / Denton, Michael L.; Eikum, Debbie M.; Noojin, Gary D.; Stolarski, David J.; Thomas, Robert J.; Glickman, Randolph D.; Rockwell, Benjamin A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / B.E. Stuck; M Belkin. Vol. 4953 2003. p. 78-84.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Denton, ML, Eikum, DM, Noojin, GD, Stolarski, DJ, Thomas, RJ, Glickman, RD & Rockwell, BA 2003, Pigmentation in NIR Laser Tissue Damage. in BE Stuck & M Belkin (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4953, pp. 78-84, Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III, San Jose,CA, United States, 1/26/03. https://doi.org/10.1117/12.476898

@inproceedings{4c379d3bcefb4fc0addd556b7e578059,

title = "Pigmentation in NIR Laser Tissue Damage",

abstract = "Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of MR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.",

keywords = "Artificial pigmentation, Cytotoxicity threshold, Fluorescence microscopy, hTERT-RPE1 cells, Mode-locked",

author = "Denton, {Michael L.} and Eikum, {Debbie M.} and Noojin, {Gary D.} and Stolarski, {David J.} and Thomas, {Robert J.} and Glickman, {Randolph D.} and Rockwell, {Benjamin A.}",

year = "2003",

doi = "10.1117/12.476898",

language = "English (US)",

volume = "4953",

pages = "78--84",

editor = "B.E. Stuck and M Belkin",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III ; Conference date: 26-01-2003 Through 27-01-2003",

}

TY - GEN

T1 - Pigmentation in NIR Laser Tissue Damage

AU - Denton, Michael L.

AU - Eikum, Debbie M.

AU - Noojin, Gary D.

AU - Stolarski, David J.

AU - Thomas, Robert J.

AU - Glickman, Randolph D.

AU - Rockwell, Benjamin A.

PY - 2003

Y1 - 2003

N2 - Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of MR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.

AB - Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of MR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.

KW - Artificial pigmentation

KW - Cytotoxicity threshold

KW - Fluorescence microscopy

KW - hTERT-RPE1 cells

KW - Mode-locked

UR - http://www.scopus.com/inward/record.url?scp=0141605827&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141605827&partnerID=8YFLogxK

U2 - 10.1117/12.476898

DO - 10.1117/12.476898

M3 - Conference contribution

AN - SCOPUS:0141605827

VL - 4953

SP - 78

EP - 84

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Stuck, B.E.

A2 - Belkin, M

T2 - Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III

Y2 - 26 January 2003 through 27 January 2003

ER -