Abstract
Damage from femtosecond mode-locked laser exposure (total exposure times longer than 20 microseconds) is predicted to be mediated by the same thermal mechanism found for continuous wave (CW) laser exposure. Experimentally, this trend holds true when comparing minimum visible lesions (MVL) from 0.25 s mode-locked and CW near-IR exposures in vivo. To further test this hypothesis in an in vitro setting we compared threshold values for cell death at various times post-exposure for mode-locked and CW 810-nm laser exposures. Using an artificially pigmented RPE cell culture system we show that damaging absorption of MR laser light is directly related to the presence of melanin pigment granules, and that the damage thresholds for mode-locked and CW laser exposures are essentially the same for the pigmented cells. We additionally conclude that the artificially pigmented RPE cell cultures are a reasonable model system for the study of thermal and photomechanical forms of ocular laser tissue damage.
Original language | English (US) |
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Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
Editors | B.E. Stuck, M Belkin |
Pages | 78-84 |
Number of pages | 7 |
Volume | 4953 |
DOIs | |
State | Published - 2003 |
Event | Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III - San Jose,CA, United States Duration: Jan 26 2003 → Jan 27 2003 |
Other
Other | Laser and Noncoherent Light Ocular Effects: Epidemiology, Prevention, and Treatment III |
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Country | United States |
City | San Jose,CA |
Period | 1/26/03 → 1/27/03 |
Keywords
- Artificial pigmentation
- Cytotoxicity threshold
- Fluorescence microscopy
- hTERT-RPE1 cells
- Mode-locked
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics