TY - JOUR
T1 - PI3Kγ deficiency enhances seizures severity and associated outcomes in a mouse model of convulsions induced by intrahippocampal injection of pilocarpine
AU - Lima, Isabel Vieira de Assis
AU - Campos, Alline Cristina
AU - Miranda, Aline Silva
AU - Vieira, Érica Leandro Marciano
AU - Amaral-Martins, Flávia
AU - Vago, Juliana Priscila
AU - Santos, Rebeca Priscila de Melo
AU - Sousa, Lirlândia Pires
AU - Vieira, Luciene Bruno
AU - Teixeira, Mauro Martins
AU - Fiebich, Bernd L.
AU - Moraes, Márcio Flávio Dutra
AU - Teixeira, Antonio Lucio
AU - de Oliveira, Antonio Carlos Pinheiro
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/5/1
Y1 - 2015/5/1
N2 - Phosphatidylinositol 3-kinase (PI3K) is an enzyme involved in different pathophysiological processes, including neurological disorders. However, its role in seizures and postictal outcomes is still not fully understood. We investigated the role of PI3Kγ on seizures, production of neurotrophic and inflammatory mediators, expression of a marker for microglia, neuronal death and hippocampal neurogenesis in mice (WT and PI3Kγ-/-) subjected to intrahippocampal microinjection of pilocarpine. PI3Kγ-/- mice presented a more severe status epilepticus (SE) than WT mice. In hippocampal synaptosomes, genetic or pharmacological blockade of PI3Kγ enhanced the release of glutamate and the cytosolic calcium concentration induced by KCl. There was an enhanced neuronal death and a decrease in the doublecortin positive cells in the dentate gyrus of PI3Kγ-/- animals after the induction of SE. Levels of BDNF were significantly increased in the hippocampus of WT and PI3Kγ-/- mice, although in the prefrontal cortex, only PI3Kγ-/- animals showed significant increase in the levels of this neurotrophic factor. Pilocarpine increased hippocampal microglial immunolabeling in both groups, albeit in the prelimbic, medial and motor regions of the prefrontal cortex this increase was observed only in PI3Kγ-/- mice. Regarding the levels of inflammatory mediators, pilocarpine injection increased interleukin (IL) 6 in the hippocampus of WT and PI3Kγ-/- animals and in the prefrontal cortex of PI3Kγ-/- animals 24h after the stimulus. Levels of TNFα were enhanced in the hippocampus and prefrontal cortex of only PI3Kγ-/- mice at this time point. On the other hand, PI3Kγ deletion impaired the increase in IL-10 in the hippocampus induced by pilocarpine. In conclusion, the lack of PI3Kγ revealed a deleterious effect in an animal model of convulsions induced by pilocarpine, suggesting that this enzyme may play a protective role in seizures and pathological outcomes associated with this condition.
AB - Phosphatidylinositol 3-kinase (PI3K) is an enzyme involved in different pathophysiological processes, including neurological disorders. However, its role in seizures and postictal outcomes is still not fully understood. We investigated the role of PI3Kγ on seizures, production of neurotrophic and inflammatory mediators, expression of a marker for microglia, neuronal death and hippocampal neurogenesis in mice (WT and PI3Kγ-/-) subjected to intrahippocampal microinjection of pilocarpine. PI3Kγ-/- mice presented a more severe status epilepticus (SE) than WT mice. In hippocampal synaptosomes, genetic or pharmacological blockade of PI3Kγ enhanced the release of glutamate and the cytosolic calcium concentration induced by KCl. There was an enhanced neuronal death and a decrease in the doublecortin positive cells in the dentate gyrus of PI3Kγ-/- animals after the induction of SE. Levels of BDNF were significantly increased in the hippocampus of WT and PI3Kγ-/- mice, although in the prefrontal cortex, only PI3Kγ-/- animals showed significant increase in the levels of this neurotrophic factor. Pilocarpine increased hippocampal microglial immunolabeling in both groups, albeit in the prelimbic, medial and motor regions of the prefrontal cortex this increase was observed only in PI3Kγ-/- mice. Regarding the levels of inflammatory mediators, pilocarpine injection increased interleukin (IL) 6 in the hippocampus of WT and PI3Kγ-/- animals and in the prefrontal cortex of PI3Kγ-/- animals 24h after the stimulus. Levels of TNFα were enhanced in the hippocampus and prefrontal cortex of only PI3Kγ-/- mice at this time point. On the other hand, PI3Kγ deletion impaired the increase in IL-10 in the hippocampus induced by pilocarpine. In conclusion, the lack of PI3Kγ revealed a deleterious effect in an animal model of convulsions induced by pilocarpine, suggesting that this enzyme may play a protective role in seizures and pathological outcomes associated with this condition.
KW - Cytokines
KW - Glutamate
KW - Microglia
KW - Neurodegeneration
KW - Neurotrophins
KW - Phosphatidylinositol 3-kinase
KW - Pilocarpine
KW - Seizures
UR - http://www.scopus.com/inward/record.url?scp=84924940526&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84924940526&partnerID=8YFLogxK
U2 - 10.1016/j.expneurol.2015.02.021
DO - 10.1016/j.expneurol.2015.02.021
M3 - Article
C2 - 25749189
AN - SCOPUS:84924940526
SN - 0014-4886
VL - 267
SP - 123
EP - 134
JO - Experimental Neurology
JF - Experimental Neurology
ER -