Physiology lab demonstration: Glomerular filtration rate in a rat

Carmen Hinojosa-Laborde, Brian Jespersen, Robert Shade

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    Measurements of glomerular filtration rate (GFR), and the fractional excretion of sodium (Na) and potassium (K) are critical in assessing renalfunction in health and disease. GFR is measured as the steady state renal clearance of inulin which is filtered at the glomerulus, but not secretedor reabsorbed along the nephron. The fractional excretion of Na and K can be determined from the concentration of Na and K in plasma andurine. The renal clearance of inulin can be demonstrated in an anesthetized animal which has catheters in the femoral artery, femoral vein andbladder. The equipment and supplies used for this procedure are those commonly available in a research core facility, and thus makes thisprocedure a practical means for measuring renal function. The purpose of this video is to demonstrate the procedures required to perform alab demonstration in which renal function is assessed before and after a diuretic drug. The presented technique can be utilized to assess renalfunction in rat models of renal disease.

    Original languageEnglish (US)
    Article numbere52425
    JournalJournal of Visualized Experiments
    Volume2015
    Issue number101
    DOIs
    StatePublished - Jul 26 2015

    Keywords

    • Blood pressure
    • Filtered load
    • Glomerular filtration rate
    • Issue 101
    • Kidney
    • Physiology
    • Potassium excretion
    • Rat
    • Sodium excretion
    • Urine flow rate

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Chemical Engineering(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Immunology and Microbiology(all)

    Fingerprint Dive into the research topics of 'Physiology lab demonstration: Glomerular filtration rate in a rat'. Together they form a unique fingerprint.

    Cite this