Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum

David Pozo; Russel J. Reiter; Juan R. Calvo; Juan M. Guerrero

doi:10.1016/0024-3205(94)00532-X

Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum

David Pozo, Russel J. Reiter, Juan R. Calvo, Juan M. Guerrero

Department of Cell Systems & Anatomy

Research output: Contribution to journal › Article › peer-review

266 Scopus citations

Abstract

In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC₅₀ value of about 0.1 mM. However, a significant inhibition of enzyme activity (> 22%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca⁺⁺. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.

Original language	English (US)
Pages (from-to)	PL455-PL460
Journal	Life Sciences
Volume	55
Issue number	24
DOIs	https://doi.org/10.1016/0024-3205(94)00532-X
State	Published - 1994

Keywords

cerebellum
melatonin
nitric oxide
pineal gland

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry, Genetics and Molecular Biology

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10.1016/0024-3205(94)00532-X

Cite this

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title = "Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum",

abstract = "In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC50 value of about 0.1 mM. However, a significant inhibition of enzyme activity (> 22\%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca++. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.",

keywords = "cerebellum, melatonin, nitric oxide, pineal gland",

author = "David Pozo and Reiter, \{Russel J.\} and Calvo, \{Juan R.\} and Guerrero, \{Juan M.\}",

note = "Funding Information: Supported by grants from CICYT (PB91-0616) and FISSS (93/0210).",

year = "1994",

doi = "10.1016/0024-3205(94)00532-X",

language = "English (US)",

volume = "55",

pages = "PL455--PL460",

journal = "Life Sciences",

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T1 - Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum

AU - Pozo, David

AU - Reiter, Russel J.

AU - Calvo, Juan R.

AU - Guerrero, Juan M.

N1 - Funding Information: Supported by grants from CICYT (PB91-0616) and FISSS (93/0210).

PY - 1994

Y1 - 1994

N2 - In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC50 value of about 0.1 mM. However, a significant inhibition of enzyme activity (> 22%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca++. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.

AB - In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC50 value of about 0.1 mM. However, a significant inhibition of enzyme activity (> 22%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca++. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.

KW - cerebellum

KW - melatonin

KW - nitric oxide

KW - pineal gland

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U2 - 10.1016/0024-3205(94)00532-X

DO - 10.1016/0024-3205(94)00532-X

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SN - 0024-3205

VL - 55

SP - PL455-PL460

JO - Life Sciences

JF - Life Sciences

IS - 24

ER -

Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum

Abstract

Keywords

ASJC Scopus subject areas

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