Physiological concentrations of melatonin inhibit nitric oxide synthase in rat cerebellum

David Pozo, Russel J. Reiter, Juan R. Calvo, Juan M. Guerrero

Research output: Contribution to journalArticle

252 Scopus citations

Abstract

In the present paper we show the inhibitory effect of melatonin on rat cerebellar nitric oxide synthase (NOS) activity. NO production was monitored by the stoichiometric conversion of L-arginine to L-citrulline. The inhibitory effect of melatonin was dose-dependent, with an IC50 value of about 0.1 mM. However, a significant inhibition of enzyme activity (> 22%) was observed at 1 nM melatonin which is in the range of the physiological serum concentration of the hormone at night. The inhibitory effect of melatonin was observed exclusively in the presence of Ca++. Results suggest a new and important role of the pineal hormone melatonin on central nervous system processes, i.e., by modulating NO production.

Original languageEnglish (US)
Pages (from-to)PL455-PL460
JournalLife Sciences
Volume55
Issue number24
DOIs
StatePublished - 1994

Keywords

  • cerebellum
  • melatonin
  • nitric oxide
  • pineal gland

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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