Physical and genetic mapping of Candida albicans: Several genes previously assigned to chromosome 1 map to chromosome R, the rDNA-containing linkage group

B. Wickes, J. Staudinger, B. B. Magee, K. J. Kwon-Chung, P. T. Magee, S. Scherer

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

Analysis of the karyotypes of multiple Candida albicans isolates by pulsed-field electrophoresis confirms the observation by Lasker et al. of eight chromosomes. The genes previously assigned to chromosome 1 in fact fall into two groups, one (including ADE1, SOR9, and CDC10) is linked to the ribosomal DNA genes on a chromosome called R, whereas the others are found on chromosome 1. Chromosome R varies in electrophoretic mobility among strains, usually running equal to or faster than chromosome 1 but in rare cases running slower than chromosome 1. In strain 1012A, the decreased mobility of one homolog is associated with the very large majority of the rDNA genes being on that homolog; the second homolog, with only a few copies, migrates with chromosome 2. Linkage analysis by using spheroplast fusion confirms the gene assignments made by hybridization to blots of the electrophoretic karyotype. A newly cloned gene, LYS2, hybridizes to chromosome 1.

Original languageEnglish (US)
Pages (from-to)2480-2484
Number of pages5
JournalInfection and immunity
Volume59
Issue number7
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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