Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells

Michael L. Denton, Debbie M. Eikum, Gary D. Noojin, David J. Stolarski, Randolph D. Glickman, Benjamin A. Rockwell

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Human retinal pigment epithelial (RPE) cells (hTERT-RPE1) were used to detect photo-oxidation products generated from chronic NIR (810 nm) laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H 2DCF-DA and its analog CM-H 2DCF-DA. Fluorescence was detected in cells exposed to mode-locked (76 MHz, ∼160 femtoseconds) but not CW laser exposure. Detection of photo-oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H 2DCF-DA dye proved a more sensitive indicator of oxidation than H 2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures (using the most sensitive fluorescent dye conditions) when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode-locked exposure.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsS.L. Jacques, W.P. Roach
Number of pages7
StatePublished - 2004
EventProgress in Biomedical Optics and Imaging - Laser Interaction with Tissue and Cells XV - San Jose, CA, United States
Duration: Jan 26 2004Jan 28 2004


OtherProgress in Biomedical Optics and Imaging - Laser Interaction with Tissue and Cells XV
Country/TerritoryUnited States
CitySan Jose, CA


  • Fluorescence microscopy
  • HTERT-RPE1 cells
  • Mode-locked laser
  • Photochemical oxidation

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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