Phosphotyrosyl-protein phosphatase of TCRC-2 cells.

G. Swarup, Kermit V Speeg, S. Cohen, D. L. Garbers

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Homogenization of TCRC-2 cells yielded a phosphotyrosyl-protein phosphatase with a specific activity approximately 10-=fold higher in particulate than in soluble fractions. Over 90% of the phosphotyrosyl-protein phosphatase associated with the particles was solubilized with 1.0% Nonidet P-40. Chromatography of the detergent-solubilized particulate fraction on either wheat germ lectin-Sepharose or histone-Sepharose columns separated two major components of phosphatase activity. One peak (eluted with 200 mM NaCl from histone-Sepharose or with N-acetylglucosamine from the lectin column) contained both phosphotyrosyl- and phosphoseryl-protein phosphatase as well as p-nitrophenyl phosphatase activities. The other peak (eluted with 1.0 M NaCl from histone-Sepharose or not bound to the lectin column) contained essentially only phosphoseryl-protein phosphatase activity. Various agents (EDTA, p-nitrophenyl phosphate, fluoride) showed considerable differences in their ability to inhibit the two phosphatase fractions; of these, the most potent and selective inhibitor was orthovanadate. At micromolar concentrations, vanadate inhibited the fraction containing phosphotyrosyl-protein phosphatase and failed to inhibit the fraction containing only phosphoseryl-protein phosphatase activity. These data show that the particulate forms of phosphotyrosyl-protein phosphatase and p-nitrophenyl phosphatase represent the activities of very similar or identical proteins.

Original languageEnglish (US)
Pages (from-to)7298-7301
Number of pages4
JournalJournal of Biological Chemistry
Volume257
Issue number13
StatePublished - Jul 10 1982
Externally publishedYes

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Protein Phosphatase 2
Protein Tyrosine Phosphatases
Sepharose
Phosphoprotein Phosphatases
4-Nitrophenylphosphatase
Histones
Vanadates
Phosphoric Monoester Hydrolases
Lectins
Wheat Germ Agglutinins
Acetylglucosamine
Chromatography
Fluorides
Edetic Acid
Detergents
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Swarup, G., Speeg, K. V., Cohen, S., & Garbers, D. L. (1982). Phosphotyrosyl-protein phosphatase of TCRC-2 cells. Journal of Biological Chemistry, 257(13), 7298-7301.

Phosphotyrosyl-protein phosphatase of TCRC-2 cells. / Swarup, G.; Speeg, Kermit V; Cohen, S.; Garbers, D. L.

In: Journal of Biological Chemistry, Vol. 257, No. 13, 10.07.1982, p. 7298-7301.

Research output: Contribution to journalArticle

Swarup, G, Speeg, KV, Cohen, S & Garbers, DL 1982, 'Phosphotyrosyl-protein phosphatase of TCRC-2 cells.', Journal of Biological Chemistry, vol. 257, no. 13, pp. 7298-7301.
Swarup G, Speeg KV, Cohen S, Garbers DL. Phosphotyrosyl-protein phosphatase of TCRC-2 cells. Journal of Biological Chemistry. 1982 Jul 10;257(13):7298-7301.
Swarup, G. ; Speeg, Kermit V ; Cohen, S. ; Garbers, D. L. / Phosphotyrosyl-protein phosphatase of TCRC-2 cells. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 13. pp. 7298-7301.
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